Galectin 9 (GAL9) ELISA Kit | MyBioSource MBS2019679 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Galectin 9 (GAL9) ELISA Kit

catalog :

MBS2019679

quantity :

48-Strip-Wells

price :

515 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2019679

products type :

ELISA Kit

products full name :

Galectin 9 (GAL9) ELISA Kit

products short name :

Galectin 9 (GAL9)

products name syn :

LGALS9; Ecalectin; Lectin,Galactoside-Binding Soluble 9; Tumor antigen HOM-HD-21

other names :

galectin-9 isoform short; Galectin-9; galectin-9; lectin, galactoside-binding, soluble, 9; Ecalectin; Tumor antigen HOM-HD-21

products gene name :

GAL9

other gene names :

LGALS9; LGALS9; HUAT; LGALS9A; Gal-9

uniprot entry name :

LEG9_HUMAN

reactivity :

Human

sequence length :

323

specificity :

This assay has high sensitivity and excellent specificity for detection of Galectin 9 (GAL9). No significant cross-reactivity or interference between Galectin 9 (GAL9) and analogues was observed.

storage stability :

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates, cell culture supernates and other biological fluids. Assay Type: Sandwich. Detection Range: 7.81-500pg/mL. Sensitivity: Typically less than 3.3pg/mL.

other info2 :

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Galectin 9 (GAL9) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Galectin 9 (GAL9) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

products description :

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Galectin 9 (GAL9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Galectin 9 (GAL9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Galectin 9 (GAL9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Galectin 9 (GAL9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

102469694

ncbi acc num :

NP_002299.2

ncbi gb acc num :

NM_002308.3

uniprot acc num :

O00182

ncbi mol weight :

35,888 Da

ncbi summary :

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. The protein encoded by this gene is an S-type lectin. It is overexpressed in Hodgkin's disease tissue and might participate in the interaction between the H RS cells with their surrounding cells and might thus play a role in the pathogenesis of this disease and/or its associated immunodeficiency. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

galectin-9: Binds galactosides. Has high affinity for the Forssman pentasaccharide. May play a role in thymocyte-epithelial interactions relevant to the biology of the thymus. Inhibits cell proliferation. It is a ligand for HAVCR2/TIM3. Induces T-helper type 1 lymphocyte (Th1) death. Isoform Short acts as an eosinophil chemoattractant. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Unknown function. Chromosomal Location of Human Ortholog: 17q11.2. Cellular Component: cytoplasm. Molecular Function: signal transducer activity; galactose binding; carbohydrate binding. Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; signal transduction

size1 :

48-Strip-Wells

price1 :

515 USD

size2 :

96-Strip-Wells

price2 :

695

size3 :

5x96-Strip-Wells

price3 :

2685

size4 :

10x96-Strip-Wells

price4 :

4850

more info or order :

MyBioSource product webpage