ELISA/assay

Cadherin 17 (CDH17) ELISA Kit

MBS2019670

48-Strip-Wells

565 USD

ELISA Kit

Cadherin 17 (CDH17) ELISA Kit

Cadherin 17 (CDH17)

CDH16; HPT-1; HPT1; LI-Cadherin; Cadherin,Liver Intestine; Intestinal peptide-associated transporter HPT-1; Liver-intestine cadherin

cadherin-17; Cadherin-17; cadherin-17; cadherin 17, LI cadherin (liver-intestine); Intestinal peptide-associated transporter HPT-1; Liver-intestine cadherin; LI-cadherin

CDH17

CDH17; CDH17; HPT1; CDH16; HPT-1; LI-cadherin

CAD17_HUMAN

Human

832

This assay has high sensitivity and excellent specificity for detection of Cadherin 17 (CDH17). No significant cross-reactivity or interference between Cadherin 17 (CDH17) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Serum, plasma, tissue homogenates, Cell lysates, cell culture supernates and other biological fluids. Assay Type: Sandwich. Detection Range: 31.25-2000pg/mL. Sensitivity: Typically less than 11.6pg/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cadherin 17 (CDH17) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cadherin 17 (CDH17) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cadherin 17 (CDH17). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cadherin 17 (CDH17). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cadherin 17 (CDH17), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Cadherin 17 (CDH17) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

221316595

NP_001138135.1

NM_001144663.1

Q12864

92,219 Da

Adherens Junctions Interactions Pathway (119533); Cell Junction Organization Pathway (160966); Cell-Cell Communication Pathway (477132); Cell-cell Junction Organization Pathway (119532)

This gene is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. The encoded protein is cadherin-like, consisting of an extracellular region, containing 7 cadherin domains, and a transmembrane region but lacking the conserved cytoplasmic domain. The protein is a component of the gastrointestinal tract and pancreatic ducts, acting as an intestinal proton-dependent peptide transporter in the first step in oral absorption of many medically important peptide-based drugs. The protein may also play a role in the morphological organization of liver and intestine. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2009]

CDH17: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. LI-cadherin may have a role in the morphological organization of liver and intestine. Involved in intestinal peptide transport. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 8q22.1. Cellular Component: basolateral plasma membrane; plasma membrane; integral to membrane. Molecular Function: transporter activity; calcium ion binding; proton-dependent oligopeptide secondary active transmembrane transporter activity. Biological Process: intercellular junction assembly and maintenance; transport; calcium-dependent cell-cell adhesion; cell adhesion; homophilic cell adhesion; oligopeptide transport

48-Strip-Wells

565 USD

96-Strip-Wells

765

5x96-Strip-Wells

2975

10x96-Strip-Wells

5340