catalog number :
MBS2019654
products type :
ELISA Kit
products full name :
Alpha-2-Glycoprotein 1, Zinc Binding (aZGP1) ELISA Kit
products short name :
[Alpha-2-Glycoprotein 1, Zinc Binding (aZGP1)]
products name syn :
[ZA2G; ZAG]
other names :
[Alpha-2-glycoprotein 1, zinc-binding; Zinc-alpha-2-glycoprotein; zinc-alpha-2-glycoprotein; alpha-2-glycoprotein 1, zinc-binding]
products gene name :
[aZGP1]
other gene names :
[AZGP1; AZGP1; ZAG; ZA2G; ZAG; ZNGP1; Zn-alpha-2-GP; Zn-alpha-2-glycoprotein]
uniprot entry name :
ZA2G_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of Alpha-2-Glycoprotein 1, Zinc Binding (aZGP1). No significant cross-reactivity or interference between Alpha-2-Glycoprotein 1, Zinc Binding (aZGP1) and analogues was observed.
storage stability :
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
image1 heading :
Typical Testing Data/Standard Curve
other info1 :
Assay Type: Double-antibody Sandwich. Samples: Serum, Plasma, Urine, Saliva, Seminal Plasma, Sweat and Other Biological Fluids. Detection Range: 4.7-300ng/mL. Sensitivity: < 1.8ng/mL
other info2 :
Application: Enzyme-linked immunosorbent assay for Antigen Detection. Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alpha-2-Glycoprotein 1, Zinc Binding (aZGP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alpha-2-Glycoprotein 1, Zinc Binding (aZGP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) =: SD/meanX100. Intra-Assay: CV<10%. Inter-Assay: CV<12%
products categories :
Metabolic pathway
products description :
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-2-Glycoprotein 1, Zinc Binding (aZGP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-2-Glycoprotein 1, Zinc Binding (aZGP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-2-Glycoprotein 1, Zinc Binding (aZGP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Alpha-2-Glycoprotein 1, Zinc Binding (aZGP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
AAH05306.1
ncbi mol weight :
34,259 Da
uniprot summary :
AZGP1: Stimulates lipid degradation in adipocytes and causes the extensive fat losses associated with some advanced cancers. May bind polyunsaturated fatty acids. Belongs to the MHC class I family. Protein type: Secreted; Secreted, signal peptide; Cell adhesion. Chromosomal Location of Human Ortholog: 7q22.1. Cellular Component: extracellular space; plasma membrane; extracellular region; MHC class I protein complex; nucleus. Molecular Function: peptide antigen binding; protein transmembrane transporter activity; glycoprotein binding; antigen binding; ribonuclease activity. Biological Process: retinal homeostasis; negative regulation of cell proliferation; antigen processing and presentation; antigen processing and presentation of peptide antigen via MHC class I; positive regulation of T cell mediated cytotoxicity; immune response; detection of chemical stimulus involved in sensory perception of bitter taste; cell adhesion
size5 :
10x96-Strip-Wells