Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1) ELISA Kit | MyBioSource MBS2019617 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1) ELISA Kit

catalog :

MBS2019617

quantity :

24-Strip-Wells

price :

270 USD

product information

catalog number :

MBS2019617

products type :

ELISA Kit

products full name :

Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1) ELISA Kit

products short name :

[Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1)]

products name syn :

[EAT; MCL1L; MCL1S; BCL2L3; TM; Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1; Bcl-2-like protein 3; Bcl-2-related protein EAT/mcl1; mcl1/EAT]

other names :

[myeloid cell leukemia sequence 1; Induced myeloid leukemia cell differentiation protein Mcl-1; induced myeloid leukemia cell differentiation protein Mcl-1; myeloid cell leukemia 1; Bcl-2-like protein 3; Bcl2-L-3; Bcl-2-related protein EAT/mcl1; mcl1/EAT]

products gene name :

[MCL1]

other gene names :

[MCL1; MCL1; TM; EAT; MCL1L; MCL1S; Mcl-1; BCL2L3; MCL1-ES; bcl2-L-3; mcl1/EAT; BCL2L3; Bcl2-L-3]

uniprot entry name :

MCL1_HUMAN

reactivity :

Human

sequence length :

350

specificity :

This assay has high sensitivity and excellent specificity for detection of Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1). No significant cross-reactivity or interference between Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1) and analogues was observed.

storage stability :

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Assay Type: Double-antibody Sandwich. Samples: Tissue homogenates, Cell lysates and Other Biological Fluids. Detection Range: 0.312-20ng/mL. Sensitivity: < 0.127ng/mL

other info2 :

Application: Enzyme-linked immunosorbent assay for Antigen Detection. Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1) were tested on 3 different plates, 8 replicates in each plate. CV(%) =: SD/meanX100. Intra-Assay: CV<10%. Inter-Assay: CV<12%

products categories :

Tumor immunity

products description :

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

4235637

ncbi acc num :

AAD13299.1

uniprot acc num :

Q07820

ncbi mol weight :

28,662 Da

ncbi pathways :

Apoptosis Pathway (198797); Apoptosis Modulation And Signaling Pathway (198822); Direct P53 Effectors Pathway (137939); E2F Transcription Factor Network Pathway (137934); HIF-1-alpha Transcription Factor Network Pathway (138045); IL-7 Signaling Pathway (198857); IL6-mediated Signaling Events Pathway (137932); MicroRNAs In Cancer Pathway (852705); MicroRNAs In Cancer Pathway (852928); PI3K-Akt Signaling Pathway (692234)

ncbi summary :

This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing. [provided by RefSeq, Oct 2010]

uniprot summary :

MCL1: a myeloid cell leukemia protein of the Bcl-2 family of proteins. Two alternatively spliced transcripts encoding distinct isoforms have been identified. The longer gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene product (isoform 2) promotes apoptosis and is death-inducing. Protein type: Apoptosis; Channel, misc.; Membrane protein, integral; Mitochondrial; Inhibitor. Chromosomal Location of Human Ortholog: 1q21. Cellular Component: nucleoplasm; mitochondrial outer membrane; mitochondrion; membrane; mitochondrial matrix; cytoplasm; integral to membrane; nucleus; cytosol. Molecular Function: protein binding; protein homodimerization activity; protein heterodimerization activity; protein channel activity; BH3 domain binding. Biological Process: apoptotic mitochondrial changes; DNA damage response, signal transduction resulting in induction of apoptosis; response to cytokine stimulus; multicellular organismal development; cell fate determination; cellular homeostasis

size1 :

24-Strip-Wells

price1 :

270 USD

size2 :

48-Strip-Wells

price2 :

495

size3 :

96-Strip-Wells

price3 :

665

size4 :

5x96-Strip-Wells

price4 :

2715

size5 :

10x96-Strip-Wells

price5 :

4895