ELISA/assay

Receptor I For The Fc Region Of Immunoglobulin G (FcgRI) ELISA Kit

MBS2019616

96 Strip Wells

745 USD

ELISA Kit

Receptor I For The Fc Region Of Immunoglobulin G (FcgRI) ELISA Kit

Receptor I For The Fc Region Of Immunoglobulin G (FcgRI)

CD64; FCGR1; FCGR1A; FCGR1B; FCGR1C; FcgRIA; CD64A; FcgRIB; CD64B; FcgRIC; CD64C; High affinity immunoglobulin gamma Fc receptor I

FcgRI, partial; FcgRI; high affinity immunoglobulin gamma Fc receptor I; FcgRI

FcgRI

LOC101087936; FcgRI

W5VJV0_FELCA

Human

296

This assay has high sensitivity and excellent specificity for detection of Receptor I For The Fc Region Of Immunoglobulin G (FcgRI). No significant cross-reactivity or interference between Receptor I For The Fc Region Of Immunoglobulin G (FcgRI) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Sample Types: Serum, plasma, tissue homogenates and other biological fluids. Detection Range: 78.13-5000pg/mL. Sensitivity: The minimum detectable dose of this kit is typically less than 36pg/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Receptor I For The Fc Region Of Immunoglobulin G (FcgRI) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Receptor I For The Fc Region Of Immunoglobulin G (FcgRI) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Receptor I For The Fc Region Of Immunoglobulin G (FcgRI). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Receptor I For The Fc Region Of Immunoglobulin G (FcgRI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Receptor I For The Fc Region Of Immunoglobulin G (FcgRI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Receptor I For The Fc Region Of Immunoglobulin G (FcgRI) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

577030171

AHH81949.1

33,486 Da

Fc Gamma R-mediated Phagocytosis Pathway 741767!!Fc Gamma R-mediated Phagocytosis Pathway 106590!!Hematopoietic Cell Lineage Pathway 741764!!Hematopoietic Cell Lineage Pathway 489!!Leishmaniasis Pathway 741865!!Leishmaniasis Pathway 144028!!Osteoclast Differentiation Pathway 741749!!Osteoclast Differentiation Pathway 193096!!Phagosome Pathway 741772!!Phagosome Pathway 153859

96 Strip Wells

745 USD