catalog number :
MBS2019512
products type :
ELISA Kit
products full name :
ELISA Kit for Involucrin (iNV)
products short name :
Involucrin (iNV)
other names :
involucrin; Involucrin; involucrin; involucrin; N/A
other gene names :
IVL; IVL; N/A
uniprot entry name :
INVO_HUMAN
storage stability :
For unopened kit, all the reagents should be kept according to the label on each vial. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C while others should be at 4 degree C.
other info1 :
Detection Range: 0.313-20ng/mL The standard curve concentrations used for the ELISA’s were 20ng/mL, 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL. Sensitivity: 0.124ng/mL
other info2 :
Assay Type: Sandwich. Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
products description :
Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to involucrin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to involucrin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain involucrin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of involucrin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
NP_005538.2
ncbi gb acc num :
NM_005547.2
ncbi mol weight :
68,479 Da
ncbi pathways :
Corticotropin-releasing Hormone Pathway 920957!!Validated Transcriptional Targets Of AP1 Family Members Fra1 And Fra2 Pathway 169349
ncbi summary :
Involucrin, a component of the keratinocyte crosslinked envelope, is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. This gene is mapped to 1q21, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin. [provided by RefSeq, Jul 2008]