ELISA/assay

Cyclophilin A (CYPA) ELISA Kit

MBS2019507

48-Strip-Wells

525 USD

ELISA Kit

Cyclophilin A (CYPA) ELISA Kit

Cyclophilin A (CYPA)

PPIA; CyP-A; CYPH; Peptidylprolyl Isomerase A; Peptidyl-Prolyl Isomerase A; Peptidyl-prolyl cis-trans isomerase A; Cyclosporin A-binding protein

cyclophilin A; cyclophilin A

CYPA

LOC100304996

Mouse

164

This assay has high sensitivity and excellent specificity for detection of hepcidin. No significant cross-reactivity or interference between hepcidin and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Assay Type: Competitive. Detection Range: 246.9-20,000pg/mL. The standard curve concentrations used for the ELISA's were 20,000pg/mL, 6,666.7pg/mL, 2,222.2pg/mL, 740.7pg/mL, 246.9pg/mL. Sensitivity: 92.5pg/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level hepcidin were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level hepcidin were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of hepcidin in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to hepcidin has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled hepcidin and unlabeled hepcidin (Standards or samples) with the pre-coated antibody specific to hepcidin. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of hepcidin in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of hepcidin in the sample.

318264336

NP_001187167.1

NM_001200238.1

48-Strip-Wells

525 USD

96-Strip-Wells

715

5x96-Strip-Wells

2760

10x96-Strip-Wells

4985