ELISA/assay

Complement Factor D (CFD) ELISA Kit

MBS2019484

48-Strip-Wells

550 USD

ELISA Kit

Complement Factor D (CFD) ELISA Kit

Complement Factor D (CFD)

CF-D; DF; PFD; ADN; Adipsin; Properdin Factor D; C3 convertase activator

complement factor D preproprotein; Complement factor D; complement factor D; complement factor D (adipsin); Adipsin; C3 convertase activator; Properdin factor D

CFD

CFD; CFD; DF; ADN; PFD; ADIPSIN; DF; PFD

CFAD_HUMAN

Mouse

253

This assay has high sensitivity and excellent specificity for detection of Complement Factor D (CFD). No significant cross-reactivity or interference between Complement Factor D (CFD) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Serum, plasma, tissue homogenates, Cell lysates, cell culture supernates and other biological fluids. Assay Type: Sandwich. Detection Range: 0.469-30ng/mL. Sensitivity: Typically less than 0.19ng/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement Factor D (CFD) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Factor D (CFD) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Factor D (CFD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Factor D (CFD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Factor D (CFD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Complement Factor D (CFD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

42544239

NP_001919.2

NM_001928.2

P00746

27,033 Da

Adipogenesis Pathway (198832); Alternative Complement Activation Pathway (106410); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Complement Cascade Pathway (106405); Hemostasis Pathway (106028); Immune System Pathway (106386); Initial Triggering Of Complement Pathway (106406); Innate Immune System Pathway (106387); Platelet Activation, Signaling And Aggregation Pathway (106034)

The protein encoded by this gene is a member of the trypsin family of peptidases. The encoded protein is a component of the alternative complement pathway best known for its role in humoral suppression of infectious agents. This protein is also a serine protease that is secreted by adipocytes into the bloodstream. Finally, the encoded protein has a high level of expression in fat, suggesting a role for adipose tissue in immune system biology. [provided by RefSeq, Jul 2008]

CFD: Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway. Defects in CFD are the cause of complement factor D deficiency (CFDD). CFDD is an immunologic disorder characterized by increased susceptibility to bacterial infections, particularly Neisseria infections, due to a defect in the alternative complement pathway. Belongs to the peptidase S1 family. Protein type: Secreted, signal peptide; Secreted; EC 3.4.21.46; Protease. Chromosomal Location of Human Ortholog: 19p13.3. Cellular Component: extracellular space; extracellular region. Molecular Function: serine-type peptidase activity; serine-type endopeptidase activity. Biological Process: platelet activation; platelet degranulation; complement activation, alternative pathway; innate immune response; proteolysis; blood coagulation; complement activation. Disease: Complement Factor D Deficiency

48-Strip-Wells

550 USD

96-Strip-Wells

750

5x96-Strip-Wells

2910

10x96-Strip-Wells

5220