ELISA/assay

Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1 (AIMP1) ELISA Kit

MBS2019478

48-Strip-Wells

625 USD

ELISA Kit

Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1 (AIMP1) ELISA Kit

Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1 (AIMP1)

SCYE1; EMAP II; EMAP2; p43; Small Inducible Cytokine Subfamily E,Member 1; Endothelial monocyte-activating polypeptide II; Multisynthase complex auxiliary component p43

aminoacyl tRNA synthase complex-interacting multifunctional protein 1 isoform a; Aminoacyl tRNA synthase complex-interacting multifunctional protein 1; aminoacyl tRNA synthase complex-interacting multifunctional protein 1; aminoacyl tRNA synthetase complex-interacting multifunctional protein 1; Multisynthase complex auxiliary component p43

AIMP1

AIMP1; AIMP1; p43; HLD3; EMAP2; SCYE1; EMAPII; EMAP2; SCYE1; EMAP-2; EMAP-II

AIMP1_HUMAN

Mouse

312

This assay has high sensitivity and excellent specificity for detection of Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1 (AIMP1). No significant cross-reactivity or interference between Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1 (AIMP1) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Serum, plasma, tissue homogenates and other biological fluids. Assay Type: Sandwich. Detection Range: 31.25-2000pg/mL. Sensitivity: Typically less than 12.4pg/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1 (AIMP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1 (AIMP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1 (AIMP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1 (AIMP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1 (AIMP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1 (AIMP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

45006986

NP_004748.2

NM_004757.3

Q12904

37,039 Da

Cytosolic TRNA Aminoacylation Pathway (105981); Gene Expression Pathway (105937); TRNA Aminoacylation Pathway (105980)

The protein encoded by this gene is a cytokine that is specifically induced by apoptosis, and it is involved in the control of angiogenesis, inflammation, and wound healing. The release of this cytokine renders the tumor-associated vasculature sensitive to tumor necrosis factor. The precursor protein is identical to the p43 subunit, which is associated with the multi-tRNA synthetase complex, and it modulates aminoacylation activity of tRNA synthetase in normal cells. This protein is also involved in the stimulation of inflammatory responses after proteolytic cleavage in tumor cells. Multiple transcript variants encoding different isoforms have been found for this gene. A pseudogene has been identified on chromosome 20. [provided by RefSeq, Dec 2008]

SCYE1 iso2: Non-catalytic component of the multisynthase complex. Stimulates the catalytic activity of cytoplasmic arginyl-tRNA synthase. Binds tRNA. Possesses inflammatory cytokine activity. Negatively regulates TGF-beta signaling through stabilization of SMURF2 by binding to SMURF2 and inhibiting its SMAD7-mediated degradation. Involved in glucose homeostasis through induction of glucagon secretion at low glucose levels. Promotes dermal fibroblast proliferation and wound repair. Regulates KDELR1- mediated retention of HSP90B1/gp96 in the endoplasmic reticulum. Plays a role in angiogenesis by inducing endothelial cell migration at low concentrations and endothelian cell apoptosis at high concentrations. Induces maturation of dendritic cells and monocyte cell adhesion. Modulates endothelial cell responses by degrading HIF-1A through interaction with PSMA7. Defects in AIMP1 are the cause of leukodystrophy hypomyelinating type 3 (HLD3). A severe autosomal recessive hypomyelinating leukodystrophy characterized by early infantile onset of global developmental delay, lack of development, lack of speech acquisition, and peripheral spasticity associated with decreased myelination in the central nervous system. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 4q24. Cellular Component: Golgi apparatus; extracellular space; aminoacyl-tRNA synthetase multienzyme complex; transport vesicle; cell surface; membrane; endoplasmic reticulum; cytoplasm; nucleus; cytosol. Molecular Function: methionine-tRNA ligase activity; protein binding; protein homodimerization activity; cytokine activity; tRNA binding. Biological Process: apoptosis; methionyl-tRNA aminoacylation; glucose metabolic process; chemotaxis; signal transduction; tRNA aminoacylation for protein translation; cell-cell signaling; negative regulation of endothelial cell proliferation; response to wounding; gene expression; angiogenesis; cell adhesion; inflammatory response; leukocyte migration. Disease: Leukodystrophy, Hypomyelinating, 3

48-Strip-Wells

625 USD

96-Strip-Wells

855

5x96-Strip-Wells

3355

10x96-Strip-Wells

6035