Apolipoprotein L (APOL1) ELISA Kit | MyBioSource MBS2019437 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Apolipoprotein L (APOL1) ELISA Kit

catalog :

MBS2019437

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2019437

products type :

ELISA Kit

products full name :

Apolipoprotein L (APOL1) ELISA Kit

products short name :

Apolipoprotein L (APOL1)

products name syn :

Apo-L; APOL; APOL-I

other names :

apolipoprotein L domain-containing protein 1 isoform 1; Apolipoprotein L domain-containing protein 1; apolipoprotein L domain-containing protein 1; apolipoprotein L domain containing 1; Vascular early response gene protein

products gene name :

APOL1

other gene names :

APOLD1; APOLD1; VERGE; VERGE

uniprot entry name :

APLD1_HUMAN

reactivity :

Human

sequence length :

279

specificity :

This assay has high sensitivity and excellent specificity for detection of APOL1. No significant cross-reactivity or interference between APOL1 and analogues was observed.

storage stability :

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

other info1 :

Samples: Human serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 0.312-20ng/mL. The standard curve concentrations used for the ELISA's were 20ng/mL, 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.312ng/mL. Sensitivity: The minimum detectable dose of APOL1 is typically less than 0.137ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level APOL1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level APOL1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products description :

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to APOL1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to APOL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain APOL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of APOL1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

194353995

ncbi acc num :

NP_001123887.1

ncbi gb acc num :

NM_001130415.1

uniprot acc num :

Q96LR9

ncbi mol weight :

27,063 Da

ncbi summary :

APOLD1 is an endothelial cell early response protein that may play a role in regulation of endothelial cell signaling and vascular function (Regard et al., 2004 [PubMed 15102925]).[supplied by OMIM, Dec 2008]

uniprot summary :

APOLD1: May be involved in angiogenesis. May play a role in activity-dependent changes of brain vasculature. May affect blood- brain permeability. Belongs to the apolipoprotein L family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Lipid-binding; Membrane protein, integral; Membrane protein, multi-pass. Chromosomal Location of Human Ortholog: 12p13.1. Cellular Component: plasma membrane; extracellular region; integral to membrane. Molecular Function: lipid binding. Biological Process: response to hypoxia; lipoprotein metabolic process; angiogenesis; cell differentiation; lipid transport; regulation of endothelial cell differentiation; endothelial cell activation

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

765

size3 :

5x96-Strip-Wells

price3 :

2975

size4 :

10x96-Strip-Wells

price4 :

5340

more info or order :

MyBioSource product webpage