ELISA/assay

High Molecular Weight Kininogen (HMWK) ELISA Kit

MBS2019405

48-Strip-Wells

540 USD

ELISA Kit

High Molecular Weight Kininogen (HMWK) ELISA Kit

High Molecular Weight Kininogen (HMWK)

Williams-Fitzgerald-Flaujeac Factor; Fitzgerald Factor; HMWK-Kallikrein Factor

high molecular weight kininogen I isoform DeltaD5; Kininogen-1; kininogen-1; kininogen 1

HMWK

Kng1; Kng1; Kng; Kng

KNG1_MOUSE

Human

480

This assay has high sensitivity and excellent specificity for detection of High Molecular Weight Kininogen (HMWK). No significant cross-reactivity or interference between High Molecular Weight Kininogen (HMWK) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Plasma. Assay Type: Sandwich. Detection Range: 0.625-40ng/mL. Sensitivity: Typically less than 0.287ng/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Molecular Weight Kininogen (HMWK) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Molecular Weight Kininogen (HMWK) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Molecular Weight Kininogen (HMWK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Molecular Weight Kininogen (HMWK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Molecular Weight Kininogen (HMWK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of High Molecular Weight Kininogen (HMWK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

50082914

AAT70087.1

O08677

53,206 Da

ACE Inhibitor Pathway (198320); Class A/1 (Rhodopsin-like Receptors) Pathway (1110499); Complement And Coagulation Cascades Pathway (198335); Complement And Coagulation Cascades Pathway (83270); Complement And Coagulation Cascades Pathway (484); Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway (1111486); Disease Pathway (1111319); Formation Of Fibrin Clot (Clotting Cascade) Pathway (1110054); G Alpha (i) Signalling Events Pathway (1110530); G Alpha (q) Signalling Events Pathway (1110532)

KNG1: (1) Kininogens are inhibitors of thiol proteases; (2) HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; (3) HMW-kininogen inhibits the thrombin- and plasmin- induced aggregation of thrombocytes; (4) the active peptide bradykinin that is released from HMW-kininogen shows a variety of physiological effects: (4A) influence in smooth muscle contraction, (4B) induction of hypotension, (4C) natriuresis and diuresis, (4D) decrease in blood glucose level, (4E) it is a mediator of inflammation and causes (4E1) increase in vascular permeability, (4E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (4F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action); (5) LMW-kininogen inhibits the aggregation of thrombocytes; (6) LMW- kininogen is in contrast to HMW-kininogen not involved in blood clotting. Defects in KNG1 are the cause of high molecular weight kininogen deficiency (HMWK deficiency). HMWK deficiency is an autosomal recessive coagulation defect. Patients with HWMK deficiency do not have a hemorrhagic tendency, but they exhibit abnormal surface-mediated activation of fibrinolysis. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Cell adhesion; Secreted; Inhibitor; Motility/polarity/chemotaxis; Contractile. Cellular Component: extracellular space; extracellular region. Molecular Function: protease inhibitor activity; cysteine protease inhibitor activity; receptor binding. Biological Process: hemostasis; negative regulation of peptidase activity; blood coagulation; inflammatory response; regulation of blood vessel size; vasodilation

48-Strip-Wells

540 USD

96-Strip-Wells

730

5x96-Strip-Wells

2830

10x96-Strip-Wells

5080