ELISA/assay

Renin (REN) ELISA Kit

MBS2019257

48-Strip-Wells

575 USD

ELISA Kit

Renin (REN) ELISA Kit

Renin (REN)

angiotensinogenase

renin preproprotein; Renin; renin; renin; Angiotensinogenase

REN

REN; REN; HNFJ2

RENI_HUMAN

Canine

406

This assay has high sensitivity and excellent specificity for detection of Renin (REN). No significant cross-reactivity or interference between Renin (REN) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Serum, plasma, tissue homogenates and other biological fluids. Assay Type: Sandwich. Detection Range: 15.63-1000pg/mL. Sensitivity: Typically less than 5.9pg/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Renin (REN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Renin (REN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Renin (REN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Renin (REN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Renin (REN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Renin (REN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

4506475

NP_000528.1

NM_000537.3

P00797

44,726 Da

ACE Inhibitor Pathway (198763); Metabolism Of Angiotensinogen To Angiotensins Pathway (685555); Metabolism Of Proteins Pathway (106230); Peptide Hormone Metabolism Pathway (771603); Renin-angiotensin System Pathway (83075); Renin-angiotensin System Pathway (486)

Renin catalyzes the first step in the activation pathway of angiotensinogen--a cascade that can result in aldosterone release,vasoconstriction, and increase in blood pressure. Renin, an aspartyl protease, cleaves angiotensinogen to form angiotensin I, which is converted to angiotensin II by angiotensin I converting enzyme, an important regulator of blood pressure and electrolyte balance. Transcript variants that encode different protein isoforms and that arise from alternative splicing and the use of alternative promoters have been described, but their full-length nature has not been determined. Mutations in this gene have been shown to cause familial hyperproreninemia. [provided by RefSeq, Jul 2008]

REN: Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. Interacts with ATP6AP2. Interaction with ATP6AP2 results in a 5-fold increased efficiency in angiotensinogen processing. Belongs to the peptidase A1 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted; EC 3.4.23.15; Protease. Chromosomal Location of Human Ortholog: 1q32. Cellular Component: extracellular space; cytoplasm; plasma membrane; extracellular region. Molecular Function: peptidase activity; insulin-like growth factor receptor binding; aspartic-type endopeptidase activity; receptor binding. Biological Process: response to cAMP; hormone-mediated signaling; drinking behavior; male gonad development; cell maturation; renin-angiotensin regulation of aldosterone production; response to lipopolysaccharide; proteolysis; angiotensin maturation; cellular protein metabolic process; regulation of blood pressure; beta-amyloid metabolic process; regulation of MAPKKK cascade; mesonephros development; kidney development. Disease: Renal Tubular Dysgenesis; Hyperuricemic Nephropathy, Familial Juvenile, 2

48-Strip-Wells

575 USD

96-Strip-Wells

785

5x96-Strip-Wells

3055

10x96-Strip-Wells

5495