Podocin (PDCN) ELISA Kit | MyBioSource MBS2019246 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Podocin (PDCN) ELISA Kit

catalog :

MBS2019246

quantity :

48-Strip-Wells

price :

550 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2019246

products type :

ELISA Kit

products full name :

Podocin (PDCN) ELISA Kit

products short name :

Podocin (PDCN)

products name syn :

NPHS2; NPHS-2; Nephrosis 2; Idiopathic Steroid-Resistant

other names :

podocin isoform 1; Podocin; podocin; nephrosis 2, idiopathic, steroid-resistant (podocin)

products gene name :

PDCN

other gene names :

NPHS2; NPHS2; PDCN; SRN1

uniprot entry name :

PODO_HUMAN

reactivity :

Rat

sequence length :

383

specificity :

This assay has high sensitivity and excellent specificity for detection of podocin. No significant cross-reactivity or interference between podocin and analogues was observed.

storage stability :

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

other info1 :

Samples: Rat tissue homogenates, cell lysates, urine, cell culture supernates and other biological fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. The standard curve concentrations used for the ELISA's were 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.312ng/mL, 0.156ng/mL. Sensitivity: 0.063ng/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level podocin were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level podocin were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products description :

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of podocin in rat tissue homogenates, cell lysates, urine, cell culture supernates and other biological fluids. Principle of the Assay The microtiter plate provided in this kit has been pre-coated with an antibody specific to podocin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to podocin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain podocin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of podocin in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

7657615

ncbi acc num :

NP_055440.1

ncbi gb acc num :

NM_014625.3

uniprot acc num :

Q9NP85

ncbi mol weight :

34,421 Da

ncbi pathways :

Nephrin/Neph1 Signaling In The Kidney Podocyte Pathway (138072)

ncbi summary :

This gene encodes a protein that plays a role in the regulation of glomerular permeability. Mutations in this gene cause steroid-resistant nephrotic syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

uniprot summary :

NPHS2: Plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton. Defects in NPHS2 are the cause of nephrotic syndrome type 2 (NPHS2). It is a renal disorder characterized clinically by childhood onset of proteinuria, hypoalbuminemia, hyperlipidemia, and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. The disorder is resistant to steroid treatment and progresses to end-stage renal failure in the first or second decades. Some patients show later onset of the disorder. Belongs to the band 7/mec-2 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 1q25.2. Cellular Component: protein complex; endoplasmic reticulum; integral to plasma membrane; plasma membrane; intercellular junction; lipid raft. Molecular Function: protein binding. Biological Process: actin cytoskeleton reorganization; excretion. Disease: Nephrotic Syndrome, Type 2

size1 :

48-Strip-Wells

price1 :

550 USD

size2 :

96-Strip-Wells

price2 :

750

size3 :

5x96-Strip-Wells

price3 :

2910

size4 :

10x96-Strip-Wells

price4 :

5220

more info or order :

MyBioSource product webpage