Brain Abundant, Membrane Attached Signal Protein 1 (BASP1) ELISA Kit | MyBioSource MBS2019218 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Brain Abundant, Membrane Attached Signal Protein 1 (BASP1) ELISA Kit

catalog :

MBS2019218

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2019218

products type :

ELISA Kit

products full name :

Brain Abundant, Membrane Attached Signal Protein 1 (BASP1) ELISA Kit

products short name :

Brain Abundant, Membrane Attached Signal Protein 1 (BASP1)

products name syn :

CAP-23; NAP-22; Brain acid soluble protein 1; 22 kDa neuronal tissue-enriched acidic protein; Neuronal axonal membrane protein NAP-22

other names :

brain abundant, membrane attached signal protein 1; Brain acid soluble protein 1; 22 kDa neuronal tissue-enriched acidic protein; Neuronal axonal membrane protein NAP-22

products gene name :

BASP1

other gene names :

BASP1; NAP22

uniprot entry name :

BASP1_HUMAN

reactivity :

Human

sequence length :

227

specificity :

This assay has high sensitivity and excellent specificity for detection of Brain Abundant, Membrane Attached Signal Protein 1 (BASP1). No significant cross-reactivity or interference between Brain Abundant, Membrane Attached Signal Protein 1 (BASP1) and analogues was observed.

storage stability :

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

other info1 :

Samples: Tissue homogenates, Cell lysates and other biological fluids. Assay Type: Sandwich. Detection Range: 0.313-20ng/mL. Sensitivity: Typically less than 0.117ng/mL.

other info2 :

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Brain Abundant, Membrane Attached Signal Protein 1 (BASP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Brain Abundant, Membrane Attached Signal Protein 1 (BASP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

products description :

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Brain Abundant, Membrane Attached Signal Protein 1 (BASP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Brain Abundant, Membrane Attached Signal Protein 1 (BASP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Brain Abundant, Membrane Attached Signal Protein 1 (BASP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Brain Abundant, Membrane Attached Signal Protein 1 (BASP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

54695552

ncbi acc num :

AAV38148.1

ncbi mol weight :

17,664 Da

uniprot summary :

BASP1: a membrane bound protein with several transient phosphorylation sites and PEST motifs. Conservation of proteins with PEST sequences among different species supports their functional significance. PEST sequences typically occur in proteins with high turnover rates. Immunological characteristics of this protein are species specific. This protein also undergoes N-terminal myristoylation. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2012]. Protein type: Adaptor/scaffold. Chromosomal Location of Human Ortholog: 5p15.1. Cellular Component: signalosome; growth cone; cytoskeleton; cytoplasm; plasma membrane; nuclear speck; cell junction; nucleus; vesicle. Molecular Function: protein domain specific binding; protein binding; transcription corepressor activity. Biological Process: substantia nigra development; gonad development; thorax and anterior abdomen determination; negative regulation of transcription, DNA-dependent

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

765

size3 :

5x96-Strip-Wells

price3 :

2975

size4 :

10x96-Strip-Wells

price4 :

5340

more info or order :

MyBioSource product webpage