ELISA/assay

FK506 Binding Protein 5 (FKBP5) ELISA Kit

MBS2019188

48-Strip-Wells

565 USD

ELISA Kit

FK506 Binding Protein 5 (FKBP5) ELISA Kit

FK506 Binding Protein 5 (FKBP5)

P54; FKBP51; FKBP54; Ptg-10; Rotamase; 51 kDa FK506-binding protein; 54 kDa progesterone receptor-associated; Androgen-regulated protein 6; HSP90-binding immunophilin

FK506 binding protein 5; Peptidyl-prolyl cis-trans isomerase FKBP5; peptidyl-prolyl cis-trans isomerase FKBP5; FK506 binding protein 5; 51 kDa FK506-binding protein; 51 kDa FKBP; FKBP-51

FKBP5

FKBP5; FKBP5; P54; AIG6; FKBP51; FKBP54; PPIase; Ptg-10; AIG6; FKBP51; PPIase FKBP5; 51 kDa FKBP; FKBP-51; FKBP-5; p54

FKBP5_HUMAN

Human

457

This assay has high sensitivity and excellent specificity for detection of FK506 Binding Protein 5 (FKBP5). No significant cross-reactivity or interference between FK506 Binding Protein 5 (FKBP5) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Serum, plasma, tissue homogenates and other biological fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: Typically less than 0.056ng/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FK506 Binding Protein 5 (FKBP5) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FK506 Binding Protein 5 (FKBP5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to FK506 Binding Protein 5 (FKBP5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to FK506 Binding Protein 5 (FKBP5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FK506 Binding Protein 5 (FKBP5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of FK506 Binding Protein 5 (FKBP5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

83404904

AAI11051.1

Q13451

30,032 Da

Estrogen Signaling Pathway (799177); Estrogen Signaling Pathway (799197); Glucocorticoid Receptor Regulatory Network Pathway (138014); Prostate Cancer Pathway (755440); TNF-alpha/NF-kB Signaling Pathway (198884)

The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This encoded protein is a cis-trans prolyl isomerase that binds to the immunosuppressants FK506 and rapamycin. It is thought to mediate calcineurin inhibition. It also interacts functionally with mature hetero-oligomeric progesterone receptor complexes along with the 90 kDa heat shock protein and P23 protein. This gene has been found to have multiple polyadenylation sites. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Mar 2009]

FKBP5: Interacts with functionally mature heterooligomeric progesterone receptor complexes along with HSP90 and TEBP. Part of a heteromultimeric cytoplasmic complex with HSP90, HSP70 and steroid receptors. Dissociates from the complex when NR3C1 binds glucocorticoid. By androgen. Widely expressed, enriched in testis compared to other tissues. Inhibited by FK506 but not cyclosporin. Protein type: Chaperone; Isomerase; EC 5.2.1.8. Chromosomal Location of Human Ortholog: 6p21.31. Cellular Component: nucleoplasm; endoplasmic reticulum membrane; membrane. Molecular Function: protein binding; peptidyl-prolyl cis-trans isomerase activity; FK506 binding; heat shock protein binding. Biological Process: protein peptidyl-prolyl isomerization; protein folding. Disease: Major Depressive Disorder

48-Strip-Wells

565 USD

96-Strip-Wells

765

5x96-Strip-Wells

2975

10x96-Strip-Wells

5340