ELISA/assay

Discs, Large Homolog 4 (DLG4) ELISA Kit

MBS2019180

48-Strip-Wells

605 USD

ELISA Kit

Discs, Large Homolog 4 (DLG4) ELISA Kit

Discs, Large Homolog 4 (DLG4)

PSD95; SAP90; Postsynaptic density protein 95; Synapse-associated protein 90

disks large homolog 4 isoform 1; Disks large homolog 4; disks large homolog 4; discs, large homolog 4 (Drosophila); Postsynaptic density protein 95; PSD-95; Synapse-associated protein 90; SAP-90; SAP90

DLG4

DLG4; DLG4; PSD95; SAP90; SAP-90; PSD95; PSD-95; SAP-90; SAP90

DLG4_HUMAN

Rat

767

This assay has high sensitivity and excellent specificity for detection of DLG4. No significant cross-reactivity or interference between DLG4 and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Rat tissue homogenates, cell lysates and other biological fluids. Assay Type: Sandwich. Detection Range: 0.312-20ng/mL. The standard curve concentrations used for the ELISA's were 20ng/mL, 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.312ng/mL. Sensitivity: The minimum detectable dose of DLG4 is typically less than 0.113ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of DLG4 in rat tissue homogenates, cell lysates and other biological fluids. Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DLG4 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DLG4 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to DLG4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to DLG4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DLG4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of DLG4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

4557529

NP_001356.1

NM_001365.3

P78352

80,125 Da

Activation Of Ca-permeable Kainate Receptor Pathway (161046); Activation Of Kainate Receptors Upon Glutamate Binding Pathway (161043); Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway (161033); Axon Guidance Pathway (105688); CREB Phosphorylation Through The Activation Of CaMKII Pathway (161040); CREB Phosphorylation Through The Activation Of Ras Pathway (161036); Cocaine Addiction Pathway (546258); Cocaine Addiction Pathway (546273); Developmental Biology Pathway (477129); ErbB4 Signaling Events Pathway (137991)

This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family. It heteromultimerizes with another MAGUK protein, DLG2, and is recruited into NMDA receptor and potassium channel clusters. These two MAGUK proteins may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signaling proteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

PSD-95: a membrane associated guanylate kinase (MAGUK) scaffolding protein located in neural postsynaptic densities. Associates with NMDA receptor NR2 subunits via glutamate serine (aspartate/glutamate) valine motifs in the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Its overexpression or depletion changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells. May reduce the amplitude of ACCN3 acid-evoked currents by retaining the channel intracellularly. Binds tissue-type plasminogen activator (tPA), mediating neural NMDA gating via a complex of LRP1, PSD-95 and NMDAR. Interacts through its first two PDZ domains with NMDAR2A, NMDAR2B, NMDAR2C, NMDAR2D, ACCN3, certain splice forms of NMDAR1, KV4.2, CXADR and synGAP. Interacts through its first two PDZ domains with Kv1.1, Kv1.2, Kv1.3, Kv1.4 and HER4. Interacts through its first PDZ domain with GluR6 and Kv1.4. Interacts through its second PDZ domain with the PDZ domain of nNOS or the C-terminus of NOS1AP. May interact with 5-HT(2A). Interacts through its third PDZ domain with NLGN1, and probably with NLGN2 and NLGN3. Interacts through its guanylate kinase-like domain with SAPAP1, SAPAP2, SAPAP3, SAPAP4, MAP1A, and BEGAIN. Interacts through its guanylate kinase-like domain with KIF13B. Two alternatively spliced human isoforms have been reported. Palmitoylation of isoform 1 is required for targeting to postsynaptic density. Protein type: Adaptor/scaffold. Chromosomal Location of Human Ortholog: 17p13.1. Cellular Component: cortical cytoskeleton; dendrite cytoplasm; voltage-gated potassium channel complex; basolateral plasma membrane; endoplasmic reticulum; postsynaptic density; dendritic spine; excitatory synapse; postsynaptic membrane; synaptic vesicle; extrinsic to internal side of plasma membrane; cytoplasm; plasma membrane; ionotropic glutamate receptor complex; synapse; cell junction. Molecular Function: protein C-terminus binding; P2Y1 nucleotide receptor binding; guanylate kinase activity; ionotropic glutamate receptor binding; protein binding; beta-1 adrenergic receptor binding; acetylcholine receptor binding; protein complex binding; protein phosphatase binding; D1 dopamine receptor binding; PDZ domain binding. Biological Process: regulation of long-term neuronal synaptic plasticity; nervous system development; axon guidance; synaptic vesicle maturation; social behavior; learning; signal transduction; positive regulation of synaptic transmission; establishment and/or maintenance of epithelial cell polarity; synaptic transmission; elevation of cytosolic calcium ion concentration; establishment of protein localization; protein complex assembly; neuromuscular process controlling balance; nucleotide phosphorylation; negative regulation of receptor internalization

48-Strip-Wells

605 USD

96-Strip-Wells

820

5x96-Strip-Wells

3220

10x96-Strip-Wells

5795