Aspartate Aminotransferase (AST) ELISA Kit | MyBioSource MBS2019147 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Aspartate Aminotransferase (AST) ELISA Kit

catalog :

MBS2019147

quantity :

24-Strip-Wells

price :

265 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2019147

products type :

ELISA Kit

products full name :

Aspartate Aminotransferase (AST) ELISA Kit

products short name :

[Aspartate Aminotransferase (AST)]

products name syn :

[cCAT; AAT; ASAT; SGOT; GOT1; Cysteine transaminase, cytoplasmic; Transaminase A; Aspartate Transaminase 1,Cytoplasmic; Glutamic-Oxaloacetic Transaminase 1,Soluble]

other names :

[aspartate aminotransferase; Aspartate aminotransferase, chloroplastic; aspartate aminotransferase; Transaminase A]

products gene name :

[AST]

other gene names :

[ASP5; ASP5; AAT3; ASP5; ASPARTATE AMINOTRANSFERASE; aspartate aminotransferase 5; ASPARTATE AMINOTRANSFERASE DEFICIENT 3; ATAAT1; F11C18.15; ]

uniprot entry name :

AAT5_ARATH

reactivity :

Mouse

sequence length :

448

specificity :

This assay has high sensitivity and excellent specificity for detection of asprosin. No significant cross-reactivity or interference between asprosin and analogues was observed.

storage stability :

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Mouse Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 78-5,000pg/mL. Sensitivity: 32pg/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level asprosin were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level asprosin were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products categories :

Enzyme Tumor immunity; Infection immunity; Hepatology

products description :

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of asprosin in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to asprosin. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to asprosin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain asprosin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of asprosin in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

334187077

ncbi acc num :

NP_001190885.1

ncbi gb acc num :

NM_001203956.1

uniprot acc num :

P46248

ncbi mol weight :

49,831 Da

ncbi pathways :

2-Oxocarboxylic Acid Metabolism Pathway (714629); 2-Oxocarboxylic Acid Metabolism Pathway (717400); Alanine, Aspartate And Glutamate Metabolism Pathway (100103); Alanine, Aspartate And Glutamate Metabolism Pathway (100063); Amino Acid Synthesis And Interconversion (transamination) Pathway (1118420); Arginine And Proline Metabolism Pathway (4017); Arginine And Proline Metabolism Pathway (323); Biosynthesis Of Amino Acids Pathway (790118); Biosynthesis Of Amino Acids Pathway (795174); Biosynthesis Of Secondary Metabolites Pathway (148695)

ncbi summary :

encodes a plastid-localized aspartate aminotransferase

uniprot summary :

Amino acid aminotransferase important for the metabolism of amino acids and Krebs-cycle related organic acids. No activity with D-Asp or D-Ala as amino donors. In plants, it is involved in nitrogen metabolism and in aspects of carbon and energy metabolism.

size1 :

24-Strip-Wells

price1 :

265 USD

size2 :

48-Strip-Wells

price2 :

490

size3 :

96-Strip-Wells

price3 :

655

size4 :

5x96-Strip-Wells

price4 :

2660

size5 :

10x96-Strip-Wells

price5 :

4795

more info or order :

MyBioSource product webpage