catalog number :
MBS2019116
products type :
ELISA Kit
products full name :
Ornithine Aminotransferase (OAT) ELISA Kit
products short name :
[Ornithine Aminotransferase (OAT)]
products name syn :
[HOGA; Grate Arophy; Ornithine delta-aminotransferase; Ornithine--oxo-acid aminotransferase]
other names :
[ornithine aminotransferase; Ornithine aminotransferase; Ornithine aminotransferase]
products gene name :
[OAT]
other gene names :
[Niako_4143]
uniprot entry name :
G8TDG9_NIAKG
specificity :
This assay has high sensitivity and excellent specificity for detection of Ornithine Aminotransferase (OAT). No significant cross-reactivity or interference between Ornithine Aminotransferase (OAT) and analogues was observed.
storage stability :
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
image1 heading :
Typical Testing Data/Standard Curve
other info1 :
Assay Type: Double-antibody Sandwich. Samples: Serum, Plasma, Tissue homogenates, Cell lysates and Other Biological Fluids. Detection Range: 0.156-10ng/mL. Sensitivity: < 0.067ng/mL
other info2 :
Application: Enzyme-linked immunosorbent assay for Antigen Detection. Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ornithine Aminotransferase (OAT) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ornithine Aminotransferase (OAT) were tested on 3 different plates, 8 replicates in each plate. CV(%) =: SD/meanX100. Intra-Assay: CV<10%. Inter-Assay: CV<12%
products categories :
Enzyme & Kinase
products description :
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ornithine Aminotransferase (OAT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ornithine Aminotransferase (OAT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ornithine Aminotransferase (OAT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Ornithine Aminotransferase (OAT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
AEW00419.1
ncbi mol weight :
44,692 Da
ncbi pathways :
Arginine And Proline Metabolism Pathway (548305); Arginine And Proline Metabolism Pathway (323); Biosynthesis Of Secondary Metabolites Pathway (548361); Metabolic Pathways (548362)
size5 :
10x96-Strip-Wells