Leucine Rich Alpha-2-Glycoprotein 1 (LRG1) ELISA Kit | MyBioSource MBS2019018 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Leucine Rich Alpha-2-Glycoprotein 1 (LRG1) ELISA Kit

catalog :

MBS2019018

quantity :

48-Strip-Wells

price :

540 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2019018

products type :

ELISA Kit

products full name :

Leucine Rich Alpha-2-Glycoprotein 1 (LRG1) ELISA Kit

products short name :

Leucine Rich Alpha-2-Glycoprotein 1 (LRG1)

other names :

leucine-rich alpha-2-glycoprotein; Leucine-rich alpha-2-glycoprotein; leucine-rich alpha-2-glycoprotein; leucine-rich alpha-2-glycoprotein 1

products gene name :

LRG1

other gene names :

LRG1; LRG1; LRG; HMFT1766; LRG; LRG

uniprot entry name :

A2GL_HUMAN

reactivity :

Human

sequence length :

347

specificity :

This assay has high sensitivity and excellent specificity for detection of Leucine Rich Alpha-2-Glycoprotein 1 (LRG1). No significant cross-reactivity or interference between Leucine Rich Alpha-2-Glycoprotein 1 (LRG1) and analogues was observed.

storage stability :

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

other info1 :

Samples: Serum, plasma, tissue homogenates and other biological fluids. Assay Type: Sandwich. Detection Range: 0.313-20ng/mL. Sensitivity: Typically less than 0.138ng/mL.

other info2 :

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Leucine Rich Alpha-2-Glycoprotein 1 (LRG1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Leucine Rich Alpha-2-Glycoprotein 1 (LRG1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

products description :

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Leucine Rich Alpha-2-Glycoprotein 1 (LRG1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Leucine Rich Alpha-2-Glycoprotein 1 (LRG1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Leucine Rich Alpha-2-Glycoprotein 1 (LRG1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Leucine Rich Alpha-2-Glycoprotein 1 (LRG1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

16418467

ncbi acc num :

NP_443204.1

ncbi gb acc num :

NM_052972.2

uniprot acc num :

P02750

ncbi mol weight :

38,178 Da

ncbi summary :

The leucine-rich repeat (LRR) family of proteins, including LRG1, have been shown to be involved in protein-protein interaction, signal transduction, and cell adhesion and development. LRG1 is expressed during granulocyte differentiation (O'Donnell et al., 2002 [PubMed 12223515]).[supplied by OMIM, Mar 2008]

uniprot summary :

LRG1: . Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 19p13.3. Cellular Component: extracellular space; membrane; extracellular region. Molecular Function: transforming growth factor beta receptor binding. Biological Process: positive regulation of angiogenesis; positive regulation of transforming growth factor beta receptor signaling pathway; brown fat cell differentiation; positive regulation of endothelial cell proliferation

size1 :

48-Strip-Wells

price1 :

540 USD

size2 :

96-Strip-Wells

price2 :

730

size3 :

5x96-Strip-Wells

price3 :

2830

size4 :

10x96-Strip-Wells

price4 :

5080

more info or order :

MyBioSource product webpage