Klotho Beta (KLb) ELISA Kit | MyBioSource MBS2019004 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Klotho Beta (KLb) ELISA Kit

catalog :

MBS2019004

quantity :

48-Strip-Wells

price :

575 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2019004

products type :

ELISA Kit

products full name :

Klotho Beta (KLb) ELISA Kit

products short name :

Klotho Beta (KLb)

products name syn :

Klotho beta-like protein

other names :

klotho beta; Beta-klotho; beta-klotho; klotho beta; Klotho beta-like protein

products gene name :

KLb

other gene names :

KLB; KLB; BKL; BKL; BetaKlotho

uniprot entry name :

KLOTB_HUMAN

reactivity :

Mouse

sequence length :

1044

specificity :

This assay has high sensitivity and excellent specificity for detection of Klotho Beta (KLb). No significant cross-reactivity or interference between Klotho Beta (KLb) and analogues was observed.

storage stability :

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

other info1 :

Samples: Serum, plasma, tissue homogenates and other biological fluids. Assay Type: Sandwich. Detection Range: 15.63-1000pg/mL. Sensitivity: Typically less than 5.7pg/mL.

other info2 :

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Klotho Beta (KLb) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Klotho Beta (KLb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

products description :

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Klotho Beta (KLb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Klotho Beta (KLb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Klotho Beta (KLb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Klotho Beta (KLb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

119613331

ncbi acc num :

EAW92925.1

uniprot acc num :

Q86Z14

ncbi mol weight :

119,808 Da

ncbi pathways :

Adaptive Immune System Pathway (366160); Constitutive PI3K/AKT Signaling In Cancer Pathway (685535); DAP12 Interactions Pathway (685549); DAP12 Signaling Pathway (685550); Disease Pathway (530764); Downstream Signal Transduction Pathway (106385); Downstream Signaling Events Of B Cell Receptor (BCR) Pathway (576250); Downstream Signaling Of Activated FGFR Pathway (160957); FGF Signaling Pathway (137989); FGFR Ligand Binding And Activation Pathway (106344)

uniprot summary :

Klotho beta: Contributes to the transcriptional repression of cholesterol 7-alpha-hydroxylase (CYP7A1), the rate-limiting enzyme in bile acid synthesis. Probably inactive as a glycosidase. Increases the ability of FGFR1 and FGFR4 to bind FGF21. Belongs to the glycosyl hydrolase 1 family. Klotho subfamily. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 4p14. Cellular Component: integral to membrane; plasma membrane. Molecular Function: protein binding; fibroblast growth factor binding; hydrolase activity, hydrolyzing O-glycosyl compounds; fibroblast growth factor receptor binding. Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; positive regulation of cell proliferation; carbohydrate metabolic process; insulin receptor signaling pathway; innate immune response

size1 :

48-Strip-Wells

price1 :

575 USD

size2 :

96-Strip-Wells

price2 :

785

size3 :

5x96-Strip-Wells

price3 :

3055

size4 :

10x96-Strip-Wells

price4 :

5495

more info or order :

MyBioSource product webpage