ELISA/assay

Angiotensin II (AngII) ELISA Kit

MBS2018983

24-Strip-Wells

265 USD

ELISA Kit

Angiotensin II (AngII) ELISA Kit

[Angiotensin II (AngII)]

[Ang-II; Angiotensin-2]

[AngII]

Rat

This assay has high sensitivity and excellent specificity for detection of Angiotensin II (AngII). No significant cross-reactivity or interference between Angiotensin II (AngII) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Standard Curve/Testing Data

Assay Type: Competitive Inhibition. Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids. Detection Range: 24.69-2,000pg/mL. Sensitivity: < 9.17pg/mL

Application: Enzyme-linked immunosorbent assay for Antigen Detection. Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Angiotensin II (AngII) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Angiotensin II (AngII) were tested on 3 different plates, 8 replicates in each plate. CV(%) =: SD/meanX100. Intra-Assay: CV<10%. Inter-Assay: CV<12%

Endocrinology; Cardiovascular biology

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Angiotensin II (AngII) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Angiotensin II (AngII) and unlabeled Angiotensin II (AngII) (Standards or samples) with the pre-coated antibody specific to Angiotensin II (AngII). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Angiotensin II (AngII) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Angiotensin II (AngII) in the sample.

24-Strip-Wells

265 USD

48-Strip-Wells

490

96-Strip-Wells

655

5x96-Strip-Wells

2660

10x96-Strip-Wells

4795