ELISA/assay

Luteinizing Hormone (LH) ELISA Kit

MBS2018978

24-Strip-Wells

265 USD

ELISA Kit

Luteinizing Hormone (LH) ELISA Kit

[Luteinizing Hormone (LH)]

[ICSH; Lutropin; Interstitial Cell Stimulating Hormone]

[LH]

Rat

This assay has high sensitivity and excellent specificity for detection of luteinizing hormone. No significant cross-reactivity or interference between luteinizing hormone and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Samples: Ovine Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Competitive. Detection Range: 370.4-30,000pg/mL. Sensitivity: 155.7pg/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level luteinizing hormone were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level luteinizing hormone were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Endocrinology; Reproductive science; Hormone metabolism

Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of luteinizing hormone in ovine serum, plasma and other biological fluids. Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to luteinizing hormone has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled luteinizing hormone and unlabeled luteinizing hormone (Standards or samples) with the pre-coated antibody specific to luteinizing hormone. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of luteinizing hormone in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of luteinizing hormone in the sample.

24-Strip-Wells

265 USD

48-Strip-Wells

490

96-Strip-Wells

655

5x96-Strip-Wells

2660

10x96-Strip-Wells

4795