ELISA/assay

Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1) ELISA Kit

MBS2018972

48-Strip-Wells

605 USD

ELISA Kit

Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1) ELISA Kit

Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1)

NMDA1; NMDAR1; NR1; Glutamate [NMDA] receptor subunit zeta-1; N-methyl-D-aspartate receptor subunit NR1

glutamate receptor ionotropic, NMDA 1 isoform GluN1-1a; Glutamate receptor ionotropic, NMDA 1; glutamate receptor ionotropic, NMDA 1; glutamate receptor, ionotropic, N-methyl D-aspartate 1; Glutamate [NMDA] receptor subunit zeta-1; N-methyl-D-aspartate receptor subunit NR1; NMD-R1

GRIN1

GRIN1; GRIN1; NR1; MRD8; GluN1; NMDA1; NMD-R1; NMDAR1; NMDAR1; GluN1; NMD-R1

NMDZ1_HUMAN

Rat

938

This assay has high sensitivity and excellent specificity for detection of GRIN1. No significant cross-reactivity or interference between GRIN1 and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Rat tissue homogenates, Cell lysates and other biological fluids. Detection Range: 1.56-100ng/mL. Sensitivity: The minimum detectable dose of GRIN1 is typically less than 0.64ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GRIN1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GRIN1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%. Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GRIN1 in rat tissue homogenates, cell lysates and other biological fluids.

Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GRIN1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to GRIN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GRIN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of GRIN1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

11038637

NP_015566.1

NM_007327.3

Q05586

101,851 Da

Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway (161033); Alcoholism Pathway (585563); Alcoholism Pathway (587116); Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Alzheimers Disease Pathway (672448); Amphetamine Addiction Pathway (547607); Amphetamine Addiction Pathway (550546); Amyotrophic Lateral Sclerosis (ALS) Pathway (83099); Amyotrophic Lateral Sclerosis (ALS) Pathway (511)

The protein encoded by this gene is a critical subunit of N-methyl-D-aspartate receptors, members of the glutamate receptor channel superfamily which are heteromeric protein complexes with multiple subunits arranged to form a ligand-gated ion channel. These subunits play a key role in the plasticity of synapses, which is believed to underlie memory and learning. Cell-specific factors are thought to control expression of different isoforms, possibly contributing to the functional diversity of the subunits. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2008]

NMDAR1: a subunit of N-methyl-D-aspartate (NMDA) receptors, members of the glutamate receptor channel superfamily. Possesses high calcium permeability and voltage-dependent sensitivity to magnesium and is modulated by glycine. Plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. Mediates neuronal functions in glutamate neurotransmission. Three alternatively-spliced isoforms have been described. Protein type: Membrane protein, integral; Channel, calcium; Membrane protein, multi-pass; Channel, ligand-gated. Chromosomal Location of Human Ortholog: 9q34.3. Cellular Component: neuron projection; cell surface; integral to plasma membrane; endoplasmic reticulum; postsynaptic density; dendrite; dendritic spine; terminal button; excitatory synapse; N-methyl-D-aspartate selective glutamate receptor complex; postsynaptic membrane; synaptic vesicle; plasma membrane; synapse; cell junction. Molecular Function: voltage-gated cation channel activity; neurotransmitter binding; glutamate receptor binding; calcium channel activity; calcium ion binding; calmodulin binding; protein binding; enzyme binding; extracellular-glutamate-gated ion channel activity; glutamate binding; protein heterodimerization activity; N-methyl-D-aspartate selective glutamate receptor activity; glycine binding. Biological Process: regulation of long-term neuronal synaptic plasticity; axon guidance; male mating behavior; prepulse inhibition; positive regulation of apoptosis; adult locomotory behavior; regulation of dendrite morphogenesis; rhythmic process; response to morphine; sensory perception of pain; regulation of axonogenesis; calcium ion homeostasis; synaptic transmission; regulation of respiratory gaseous exchange; conditioned taste aversion; ephrin receptor signaling pathway; visual learning; negative regulation of neuron apoptosis; protein tetramerization; cation transport; synaptic transmission, glutamatergic; response to amphetamine; respiratory gaseous exchange; social behavior; pons maturation; cellular calcium ion homeostasis; regulation of membrane potential; response to ethanol; regulation of synaptogenesis; propylene metabolic process; long-term memory; olfactory learning; suckling behavior; ionotropic glutamate receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; cerebral cortex development; response to calcium ion; regulation of excitatory postsynaptic membrane potential. Disease: Mental Retardation, Autosomal Dominant 8

48-Strip-Wells

605 USD

96-Strip-Wells

820

5x96-Strip-Wells

3220

10x96-Strip-Wells

5795