ELISA/assay

Junction Plakoglobin (JUP) ELISA Kit

MBS2018947

24-Strip-Wells

270 USD

ELISA Kit

Junction Plakoglobin (JUP) ELISA Kit

[Junction Plakoglobin (JUP)]

[DP3; gCat; G-Cat; CTNNG; DPIII; PDGB; PKGB; Gamma Catenin; Desmoplakin III; Desmoplakin-3]

[junction plakoglobin; Junction plakoglobin; junction plakoglobin; junction plakoglobin; Catenin gamma; Desmoplakin III; Desmoplakin-3]

[JUP]

[JUP; JUP; DP3; PDGB; PKGB; CTNNG; DPIII; ARVD12; CTNNG; DP3]

PLAK_HUMAN

Human

745

This assay has high sensitivity and excellent specificity for detection of ITGb1. No significant cross-reactivity or interference between ITGb1 and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Samples: Human Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.78-50ng/mL. Sensitivity: 0.31ng/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ITGb1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ITGb1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

CD & Adhesion molecule

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ITGb1 in human tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to ITGb1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to ITGb1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ITGb1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ITGb1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

12056468

NP_068831.1

NM_021991.2

P14923

81,745 Da

Acute Myeloid Leukemia Pathway (83117); Acute Myeloid Leukemia Pathway (529); Adherens Junctions Interactions Pathway (119533); Arf6 Trafficking Events Pathway (137954); Arrhythmogenic Right Ventricular Cardiomyopathy Pathway (672454); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (117293); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (116129); Cell Junction Organization Pathway (160966); Cell-Cell Communication Pathway (477132); Cell-cell Junction Organization Pathway (119532)

This gene encodes a major cytoplasmic protein which is the only known constituent common to submembranous plaques of both desmosomes and intermediate junctions. This protein forms distinct complexes with cadherins and desmosomal cadherins and is a member of the catenin family since it contains a distinct repeating amino acid motif called the armadillo repeat. Mutation in this gene has been associated with Naxos disease. Alternative splicing occurs in this gene; however, not all transcripts have been fully described. [provided by RefSeq, Jul 2008]

CTNNG: Common junctional plaque protein. The membrane- associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE- cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton. Homodimer. Component of an E-cadherin/ catenin adhesion complex composed of at least E-cadherin/CDH1 and gamma- catenin/JUP, and possibly alpha-catenin/CTNNA1; the complex is located to adherens junctions. The stable association of CTNNA1 is controversial as CTNNA1 was shown not to bind to F-actin when assembled in the complex. Interacts with MUC1. Interacts with CAV1. Interacts with PTPRJ. Interacts with DSC2. Belongs to the beta-catenin family. Protein type: Motility/polarity/chemotaxis; Cytoskeletal. Chromosomal Location of Human Ortholog: 17q21. Cellular Component: desmosome; internal side of plasma membrane; focal adhesion; apicolateral plasma membrane; intermediate filament; zonula adherens; catenin complex; intercellular junction; fascia adherens; Z disc; cytosol; actin cytoskeleton; cell-cell adherens junction; cytoskeleton; hemidesmosome; cytoplasm; plasma membrane; nucleus; lateral plasma membrane. Molecular Function: protein binding; signal transducer activity; protein homodimerization activity; structural constituent of cell wall; cadherin binding; transcription coactivator activity; structural molecule activity; protein kinase binding; protein phosphatase binding; nuclear hormone receptor binding; alpha-catenin binding. Biological Process: skin development; intercellular junction assembly and maintenance; cell-cell adhesion; cell migration; positive regulation of protein import into nucleus; cytoskeletal anchoring; protein heterooligomerization; regulation of cell fate specification; detection of mechanical stimulus; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; vascular endothelial growth factor receptor signaling pathway; regulation of cell proliferation. Disease: Arrhythmogenic Right Ventricular Dysplasia, Familial, 12; Naxos Disease

24-Strip-Wells

270 USD

48-Strip-Wells

495

96-Strip-Wells

665

5x96-Strip-Wells

2715

10x96-Strip-Wells

4895