ELISA/assay

Solute Carrier Family 16, Member 1 (SLC16A1) ELISA Kit

MBS2018920

24-Strip-Wells

270 USD

ELISA Kit

Solute Carrier Family 16, Member 1 (SLC16A1) ELISA Kit

[Solute Carrier Family 16, Member 1 (SLC16A1)]

[MCT1; MCT; Monocarboxylate Transporter 1; Monocarboxylic Acid Transporter 1]

[solute carrier family 16 (monocarboxylic acid transporters), member 1, isoform CRA_b; Monocarboxylate transporter 1; monocarboxylate transporter 1; solute carrier family 16 (monocarboxylate transporter), member 1; Solute carrier family 16 member 1]

[SLC16A1]

[SLC16A1; SLC16A1; MCT; HHF7; MCT1; MCT1D; MCT1; MCT 1]

MOT1_HUMAN

Human

500

This assay has high sensitivity and excellent specificity for detection of sIgA. No significant cross-reactivity or interference between sIgA and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Samples: Rat Serum, Plasma, Saliva And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 6.25-400ng/mL. Sensitivity: 2.64ng/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level sIgA were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level sIgA were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Signal transduction

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of sIgA in rat serum, plasma, saliva and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to sIgA. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to sIgA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain sIgA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of sIgA in the samples is then determined by comparing the O.D. of the samples to the standard curve.

119576957

EAW56553.1

P53985

46,234 Da

Basigin Interactions Pathway (106065); Cell Surface Interactions At The Vascular Wall Pathway (106062); Hemostasis Pathway (106028); Metabolism Pathway (477135); Proton-coupled Monocarboxylate Transport Pathway (161065); Pyruvate Metabolism Pathway (106205); Pyruvate Metabolism And Citric Acid (TCA) Cycle Pathway (106327); SLC-mediated Transmembrane Transport Pathway (119558); The Citric Acid (TCA) Cycle And Respiratory Electron Transport Pathway (477137); Transmembrane Transport Of Small Molecules Pathway (106572)

The protein encoded by this gene is a proton-linked monocarboxylate transporter that catalyzes the movement of many monocarboxylates, such as lactate and pyruvate, across the plasma membrane. Mutations in this gene are associated with erythrocyte lactate transporter defect. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Oct 2009]

MCT1: Proton-linked monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate. Defects in SLC16A1 are the cause of symptomatic deficiency in lactate transport (SDLT); also known as erythrocyte lactate transporter defect. Deficiency of lactate transporter may result in an acidic intracellular environment created by muscle activity with consequent degeneration of muscle and release of myoglobin and creatine kinase. This defect might compromise extreme performance in otherwise healthy individuals. Defects in SLC16A1 are the cause of familial hyperinsulinemic hypoglycemia type 7 (HHF7); also known as exercise-induced hyperinsulinemic hypoglycemia. HHF7 is a dominantly inherited hypoglycemic disorder characterized by inappropriate insulin secretion during anaerobic exercise or on pyruvate load. Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family. Protein type: Membrane protein, multi-pass; Transporter, SLC family; Transporter; Membrane protein, integral; Mitochondrial. Chromosomal Location of Human Ortholog: 1p12. Cellular Component: centrosome; membrane; mitochondrion; integral to plasma membrane; integral to membrane; plasma membrane. Molecular Function: mevalonate transmembrane transporter activity; protein homodimerization activity; monocarboxylic acid transmembrane transporter activity; symporter activity; lactate transmembrane transporter activity; secondary active monocarboxylate transmembrane transporter activity. Biological Process: response to food; cellular metabolic process; monocarboxylic acid transport; centrosome organization and biogenesis; mevalonate transport; lipid metabolic process; blood coagulation; pyruvate metabolic process; glucose homeostasis; leukocyte migration; transmembrane transport; regulation of insulin secretion; behavioral response to nutrient. Disease: Erythrocyte Lactate Transporter Defect; Monocarboxylate Transporter 1 Deficiency; Hyperinsulinemic Hypoglycemia, Familial, 7

24-Strip-Wells

270 USD

48-Strip-Wells

495

96-Strip-Wells

665

5x96-Strip-Wells

2715

10x96-Strip-Wells

4895