Coagulation Factor VIII (F8) ELISA Kit | MyBioSource MBS2018854 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Coagulation Factor VIII (F8) ELISA Kit

catalog :

MBS2018854

quantity :

24-Strip-Wells

price :

260 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2018854

products type :

ELISA Kit

products full name :

Coagulation Factor VIII (F8) ELISA Kit

products short name :

[Coagulation Factor VIII (F8)]

products name syn :

[FVIII; AHF; HEMA; F8B; F8C; Antihemophilic Globulin; Hemophilia A; Anti-Hemophilic Factor A; FVIII Procoagulant Component; F8 Protein]

other names :

[coagulation factor VIII isoform 1; Coagulation factor VIII; coagulation factor VIII; coagulation factor VIII; Procoagulant component]

products gene name :

[F8]

other gene names :

[F8; F8; Cf8; Cf-8; FVIII; Cf8; F8c]

uniprot entry name :

FA8_MOUSE

reactivity :

Human

sequence length :

2319

specificity :

This assay has high sensitivity and excellent specificity for detection of F2. No significant cross-reactivity or interference between F2 and analogues was observed.

storage stability :

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Porcine Serum. Assay Type: Quantitative Sandwich. Detection Range: 3.12-200ng/mL. Sensitivity: 1.13ng/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level F2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level F2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products categories :

Metabolic pathway; Hematology

products description :

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of F2 in porcine serum. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to F2. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to F2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain F2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of F2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

238624180

ncbi acc num :

NP_032003.2

ncbi gb acc num :

NM_007977.2

uniprot acc num :

Q06194

ncbi mol weight :

266,196 Da

ncbi pathways :

Blood Clotting Cascade Pathway (198388); Common Pathway (1110057); Complement And Coagulation Cascades Pathway (198335); Complement And Coagulation Cascades Pathway (83270); Complement And Coagulation Cascades Pathway (484); Formation Of Fibrin Clot (Clotting Cascade) Pathway (1110054); Hemostasis Pathway (1110026); Intrinsic Pathway (1110056); Platelet Activation, Signaling And Aggregation Pathway (1110036); Platelet Degranulation Pathway (1110053)

uniprot summary :

F8: Factor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa. Defects in F8 are the cause of hemophilia A (HEMA). A disorder of blood coagulation characterized by a permanent tendency to hemorrhage. About 50% of patients have severe hemophilia resulting in frequent spontaneous bleeding into joints, muscles and internal organs. Less severe forms are characterized by bleeding after trauma or surgery. Of particular interest for the understanding of the function of F8 is the category of CRM (cross-reacting material) positive patients (approximately 5%) that have considerable amount of F8 in their plasma (at least 30% of normal), but the protein is non- functional; i.e. the F8 activity is much less than the plasma protein level. CRM-reduced is another category of patients in which the F8C antigen and activity are reduced to approximately the same level. Most mutations are CRM negative, and probably affect the folding and stability of the protein. Belongs to the multicopper oxidase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted. Cellular Component: extracellular space; extracellular region. Molecular Function: copper ion binding; metal ion binding; serine-type endopeptidase activity; oxidoreductase activity. Biological Process: platelet activation; hemostasis; acute-phase response; blood coagulation; proteolysis; blood coagulation, intrinsic pathway

size1 :

24-Strip-Wells

price1 :

260 USD

size2 :

48-Strip-Wells

price2 :

475

size3 :

96-Strip-Wells

price3 :

640

size4 :

5x96-Strip-Wells

price4 :

2590

size5 :

10x96-Strip-Wells

price5 :

4670

more info or order :

MyBioSource product webpage