catalog number :
MBS2018828
products type :
Recombinant Protein
products full name :
Recombinant Spondin 1 (SPON1)
products short name :
[Spondin 1 (SPON1)]
other names :
[spondin-1; Spondin-1; spondin-1; spondin 1, (f-spondin) extracellular matrix protein; vascular smooth muscle cell growth-promoting factor; spondin 1, extracellular matrix protein; F-spondin; Vascular smooth muscle cell growth-promoting factor]
products gene name :
[SPON1]
other gene names :
[SPON1; SPON1; f-spondin; VSGP/F-spondin; KIAA0762; VSGP]
uniprot entry name :
SPON1_HUMAN
sequence :
The sequence of the target protein is listed below. AETS QAEKCMMPEC HTIPCLLSPW SEWSDCSVTC GKGMRTRQRM LKSLAELGDC NEDLEQVEKC MLPECPIDCE LTEWSQWSEC NKSCGKGHVI RTRMIQMEPQ FGGAPCPETV QRKKCRIRKC LRNPSIQKLR WREARESRRS EQLKEESEGE QFPGCRMRPW TAWSECTKLC GGGIQERYMT VKKRFKSSQF TSCKDKKEIR A
form :
Supplied as lyophilized form in 100mM NaHCO3, 500mM NaCl, pH8.3, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
storage stability :
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
tested application :
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
image1 heading :
SDS-Page
other info1 :
Organism: Homo sapiens (Human). Expression System: Prokaryotic expression. Residues: Ala597~Ala801 (Accession # Q9HCB6) with N-terminal His-Tag. Subcellular Location: Secreted, extracellular space, extracellular matrix. Predicted isoelectric point: 8.7. Predicted Molecular Mass: 25.1kDa
other info2 :
Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Reconstitution: Reconstitute in sterile ddH2O.
ncbi acc num :
NP_006099.2
ncbi gb acc num :
NM_006108.3
uniprot summary :
SPON1: Cell adhesion protein that promotes the attachment of spinal cord and sensory neuron cells and the outgrowth of neurites in vitro. May contribute to the growth and guidance of axons in both the spinal cord and the PNS. Major factor for vascular smooth muscle cell. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 11p15.2. Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; endoplasmic reticulum lumen. Molecular Function: protein binding. Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; cell adhesion; post-translational protein modification