Recombinant Heat Shock 70kDa Protein 9 (HSPA9) | MyBioSource MBS2018822 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Heat Shock 70kDa Protein 9 (HSPA9)

catalog :

MBS2018822

quantity :

0.01 mg

price :

185 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2018822

products type :

Recombinant Protein

products full name :

Recombinant Heat Shock 70kDa Protein 9 (HSPA9)

products short name :

Heat Shock 70kDa Protein 9 (HSPA9)

other names :

stress-70 protein, mitochondrial; Stress-70 protein, mitochondrial; stress-70 protein, mitochondrial; mortalin-2; p66-mortalin; mortalin, perinuclear; heat shock 70kD protein 9B; peptide-binding protein 74; 75 kDa glucose-regulated protein; heat shock 70kDa protein 9 (mortalin); 75 kDa glucose-regulated protein; GRP-75; Heat shock 70 kDa protein 9; Mortalin; MOT; Peptide-binding protein 74

products gene name :

HSPA9

other gene names :

HSPA9; HSPA9; CSA; MOT; MOT2; GRP75; PBP74; GRP-75; HSPA9B; MTHSP75; GRP75; HSPA9B; mt-HSP70; GRP-75; MOT; PBP74

uniprot entry name :

GRP75_HUMAN

host :

E Coli

sequence length :

679

sequence :

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- SCVAVM EGKQAKVLEN AEGARTTPSV VAFTADGERL VGMPAKRQAV TNPNNTFYAT KRLIGRRYDD PEVQKDIKNV PFKIVRASNG DAWVEAHGKL YSPSQIGAFV LMKMKETAEN YLGHTAKNAV ITVPAYFNDS QRQATKDAGQ ISGLNVLRVI NEPTAAALAY GLDKSEDKVI AVYDLGGGTF DISILEIQKGVFEVKSTNGD TFLGGEDFDQ ALLRHIVKEF KRETGVDLTK DNMALQRVRE A

purity :

> 95%

form :

Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH 8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.

storage stability :

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

tested application :

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

other info1 :

Organism: Homo sapiens (Human). Expression System: Prokaryotic expression. Residues: Ser65~Ala311 (Accession # P38646) with two N-terminal Tags, His-tag and T7-tag. Subcellular Location: Mitochondrion. Nucleus, nucleolus

other info2 :

Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Predicted Isoelectric Point: 7.9. Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

products description :

About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.

ncbi gi num :

24234688

ncbi acc num :

NP_004125.3

ncbi gb acc num :

NM_004134.6

uniprot acc num :

P38646

ncbi mol weight :

30.8kDa

ncbi pathways :

Metabolism Of Proteins Pathway (106230); Mitochondrial Protein Import Pathway (576261); Parkin-Ubiquitin Proteasomal System Pathway (700638); RNA Degradation Pathway (117291); RNA Degradation Pathway (116127); Tuberculosis Pathway (213780); Tuberculosis Pathway (213743)

ncbi summary :

This gene encodes a member of the heat shock protein 70 gene family. The encoded protein is primarily localized to the mitochondria but is also found in the endoplasmic reticulum, plasma membrane and cytoplasmic vesicles. This protein is a heat-shock cognate protein. This protein plays a role in cell proliferation, stress response and maintenance of the mitochondria. A pseudogene of this gene is found on chromosome 2.[provided by RefSeq, May 2010]

uniprot summary :

HSPA9B: Implicated in the control of cell proliferation and cellular aging. May also act as a chaperone. Interacts with FXN. Interacts with HSCB. Component of the MINOS/MitOS complex, that includes IMMT, HSPA9 and CHCHD3 and associates with mitochondrial outer membrane proteins SAMM50, MTX1 and MTX2. Belongs to the heat shock protein 70 family. Protein type: Heat shock protein; Chaperone; Nucleolus. Chromosomal Location of Human Ortholog: 5q31.1. Cellular Component: focal adhesion; mitochondrion; cytoplasm; nucleolus. Molecular Function: protein binding; ubiquitin protein ligase binding; unfolded protein binding; ATP binding. Biological Process: cellular protein metabolic process; protein folding; protein export from nucleus; protein targeting to mitochondrion; negative regulation of apoptosis

size1 :

0.01 mg

price1 :

185 USD

size2 :

0.05 mg

price2 :

385

size3 :

0.1 mg

price3 :

595

more info or order :

MyBioSource product webpage