Recombinant Fucosidase Alpha L1, Tissue (FUCa1) | MyBioSource MBS2018787 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Fucosidase Alpha L1, Tissue (FUCa1)

catalog :

MBS2018787

quantity :

0.01 mg

price :

145 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2018787

products type :

Recombinant Protein

products full name :

Recombinant Fucosidase Alpha L1, Tissue (FUCa1)

products short name :

[Fucosidase Alpha L1, Tissue (FUCa1)]

other names :

[tissue alpha-L-fucosidase; Tissue alpha-L-fucosidase; tissue alpha-L-fucosidase; alpha-L-fucosidase 1; alpha-L-fucosidase I; fucosidase, alpha-L-1, tissue; alpha-L-fucoside fucohydrolase 1; fucosidase, alpha-L- 1, tissue; Alpha-L-fucosidase I; Alpha-L-fucoside fucohydrolase 1]

products gene name :

[FUCa1]

other gene names :

[Fuca1; Fuca1; Afuc; Fuca; 0610006A03Rik; 9530055J05Rik; Fuca; Alpha-L-fucosidase 1]

uniprot entry name :

FUCO_MOUSE

host :

E Coli

sequence length :

452

sequence :

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- NGFKTQ HFVRAKTMPE LYDLVNSYKP DLIWSDGEWE CPDTYWNSTS FLAWLYNDSP VKDEVIVNDR WGQNCSCHHG GYYNCQDKYK PQSLPDHKWE MCTSMDRASW GYRKDMTMST IAKENEIIEE LVQTVSLGGN YLLNIGPTKD GLIVPIFQER LLAVGKWLQI NGEAIYASKP WRVQSEKNKT VVWYTTKNAT VYATFLYWPE NGIVNLKSPK TTSATKITML GLEGDLSWTQDPLEGVLISL PQLPPTVLPV EFAWTLK

purity :

> 95%

form :

Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.

storage stability :

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

tested application :

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

image1 heading :

SDS-Page

other info1 :

Organism: Mus musculus (Mouse). Expression System: Prokaryotic expression. Residues: Asn185~Lys447 (Accession # Q99LJ1) with two N-terminal Tags, His-tag and T7-tag. Subcellular Location: Lysosome

other info2 :

Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Predicted Isoelectric Point: 6.3. Reconstitution: Reconstitute in sterile ddH2O.

products description :

About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.

ncbi gi num :

169808427

ncbi acc num :

NP_077205.3

ncbi gb acc num :

NM_024243.4

uniprot acc num :

Q99LJ1

ncbi mol weight :

34.0kDa

ncbi pathways :

Lysosome Pathway (99272); Lysosome Pathway (96865); Other Glycan Degradation Pathway (83175); Other Glycan Degradation Pathway (346)

uniprot summary :

FUCA1: Alpha-L-fucosidase is responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N- acetylglucosamine of the carbohydrate moieties of glycoproteins. Defects in FUCA1 are the cause of fucosidosis (FUCA1D). FUCA1D is an autosomal recessive lysosomal storage disease characterized by accumulation of fucose-containing glycolipids and glycoproteins in various tissues. Clinical signs include facial dysmorphism, dysostosis multiplex, moderate hepatomegaly, severe intellectual deficit, deafness, and according to age, angiokeratomas. Belongs to the glycosyl hydrolase 29 family. Protein type: Glycan Metabolism - other glycan degradation; EC 3.2.1.51; Hydrolase. Cellular Component: lysosome. Molecular Function: fucosidase activity; hydrolase activity; alpha-L-fucosidase activity; hydrolase activity, acting on glycosyl bonds; carbohydrate binding; fucose binding. Biological Process: fucose metabolic process; glycoside catabolic process; metabolic process; carbohydrate metabolic process

size1 :

0.01 mg

price1 :

145 USD

size2 :

0.05 mg

price2 :

280

size3 :

0.1 mg

price3 :

440

size4 :

0.2 mg

price4 :

540

size5 :

0.5 mg

price5 :

1040

size6 :

1 mg

price6 :

1545

more info or order :

MyBioSource product webpage