Recombinant Laminin Alpha 5 (LAMa5) | MyBioSource MBS2018755 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Laminin Alpha 5 (LAMa5)

catalog :

MBS2018755

quantity :

0.01 mg

price :

190 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2018755

products type :

Recombinant Protein

products full name :

Recombinant Laminin Alpha 5 (LAMa5)

products short name :

Laminin Alpha 5 (LAMa5)

other names :

laminin subunit alpha-5; Laminin subunit alpha-5; laminin subunit alpha-5; laminin-10 subunit alpha; laminin-11 subunit alpha; laminin-15 subunit alpha; laminin, alpha 5; Laminin-10 subunit alpha; Laminin-11 subunit alpha; Laminin-15 subunit alpha

products gene name :

LAMa5

other gene names :

Lama5; Lama5; [a]5; AA408760; AA408762; AI853660; mKIAA0533; laminin-511

uniprot entry name :

LAMA5_MOUSE

host :

E Coli

sequence length :

3718

sequence :

The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSG SEF- EDGL FFPGSEGVVT LELPKAKMPY VSLELEMRPL AAAGLIFHLG QALATPYMQL KVLTEQVLLQ ANDGAGEFST WVTYPKLCDG RWHRVAVIMG RDTLRLEVDT QSNHTTGRLP ESLAGSPALL HLGSLPKSST ARPELPAYRG CLRKLLINGA PVNVTASVQIQGAVGMRGCP SGTLALSKQG KALTQRQAKP SVSPLLWH

purity :

> 95%

form :

Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.

storage stability :

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

tested application :

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

other info1 :

Organism: Mus musculus (Mouse). Expression System: Prokaryotic expression. Residues: Glu3517~His3718 (Accession # Q61001) with N-terminal His-Tag. Subcellular Location: Secreted, extracellular space, extracellular matrix, basement membrane. Predicted isoelectric point: 9.1 . Accurate Molecular Mass: 26kDa as determined by SDS-PAGE reducing conditions. Usage: Reconstitute in ddH2O

other info2 :

Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4. Accurate Molecular Mass: 27kDa as determined by SDS-PAGE reducing conditions. Storage Buffer: 62.5mM Tris-H3PO4 (pH 7.5 at 25 degree C), 1mM EDTA, 2% (w/v) SDS, 100mM DTT, 1mM NaN3, 0.01% (w/v) bromo-phenol blue and 33% (v/v) glycerol.

products description :

About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.

ncbi gi num :

124487155

ncbi acc num :

NP_001074640.1

ncbi gb acc num :

NM_001081171.2

uniprot acc num :

Q61001

ncbi mol weight :

23.2kDa

ncbi pathways :

Alpha6-Beta4 Integrin Signaling Pathway (198373); Amoebiasis Pathway (167330); Amoebiasis Pathway (167191); Degradation Of The Extracellular Matrix Pathway (927074); ECM Proteoglycans Pathway (927073); ECM-receptor Interaction Pathway (83265); ECM-receptor Interaction Pathway (479); Extracellular Matrix Organization Pathway (927061); Focal Adhesion Pathway (198353); Focal Adhesion Pathway (83264)

uniprot summary :

LAMA5: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Protein type: Secreted; Motility/polarity/chemotaxis; Extracellular matrix; Secreted, signal peptide. Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; laminin-5 complex; laminin-1 complex; laminin-10 complex; extracellular region; basement membrane; basal lamina; nucleus. Molecular Function: integrin binding; protein binding; receptor binding. Biological Process: integrin-mediated signaling pathway; regulation of cell adhesion; cell migration; muscle development; regulation of embryonic development; regulation of cell migration; odontogenesis of dentine-containing teeth; regulation of cell proliferation; organ morphogenesis; morphogenesis of embryonic epithelium; branching morphogenesis of a tube; hair follicle development; ureteric bud branching; cilium biogenesis; cell adhesion; neural crest cell migration; morphogenesis of a polarized epithelium; lung development

size1 :

0.01 mg

price1 :

190 USD

size2 :

0.05 mg

price2 :

395

size3 :

0.1 mg

price3 :

610

more info or order :

MyBioSource product webpage