protein

Recombinant Cartilage Intermediate Layer Protein (CILP)

MBS2018653

0.01 mg

185 USD

Recombinant Protein

Recombinant Cartilage Intermediate Layer Protein (CILP)

Cartilage Intermediate Layer Protein (CILP)

cartilage intermediate layer protein 1; Cartilage intermediate layer protein 1; cartilage intermediate layer protein 1; CILP-1; cartilage intermediate layer protein, nucleotide pyrophosphohydrolase

CILP

Cilp; Cilp; 9830114D16; C130036G17Rik; CILP-1

CILP1_MOUSE

E Coli

1250

The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below. MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-PSKSFYR QNGEPFTGKV KASVTFLDPR NISTATAAQS DLNFINDEGD TFPLRTYGMF SVDFRDEATS ESLNAGKVKV HLDSTQVKMP EHVPAMKLWS LNPDTGLWEE EGDFKFESQR RNKREERTFL VGNMEIRERR LFNLDVPESR RCFIKVRTYR SERFLPSEQI QGVVVSVINL EPRTGFSSNP RAWGRFDSVI TGPNGACLPA FCDDQSPDAY SVYVLASLSG EELEAVESSP KFNPNAIGVP QPYLNKLKYR RTDH

> 95%

Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

Organism: Mus musculus (Mouse). Expression System: Prokaryotic expression. Residues: Pro604~His864 (Accession # Q66K08) with two N-terminal Tags, His-tag and T7-tag. Subcellular Location: Secreted, extracellular space, extracellular matrix

Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Predicted Isoelectric Point: 6.3. Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.

27734196

NP_775561.1

NM_173385.2

Q66K08

62.0 kDa

CILP: Probably plays a role in cartilage scaffolding. May act by antagonizing TGF-beta1 (TGFB1) and IGF1 functions. Has the ability to suppress IGF1-induced proliferation and sulfated proteoglycan synthesis, and inhibits ligand-induced IGF1R autophosphorylation. May inhibit TGFB1-mediated induction of cartilage matrix genes via its interaction with TGFB1. Overexpression may lead to impair chondrocyte growth and matrix repair and indirectly promote inorganic pyrophosphate (PPi) supersaturation in aging and osteoarthritis cartilage. Defects in CILP are a cause of susceptibility to intervertebral disk disease (IDD). A common musculo- skeletal disorder caused by degeneration of intervertebral disks of the lumbar spine. It results in low-back pain and unilateral leg pain. Susceptibility to intervertebral disk disease, is conferred by variant p.Ile395Thr (PubMed:15864306). Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted. Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; extracellular region. Biological Process: negative regulation of insulin-like growth factor receptor signaling pathway

0.01 mg

185 USD

0.05 mg

395

0.1 mg

605