Recombinant Cytochrome P450 24A1 (CYP24A1) | MyBioSource MBS2018602 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Cytochrome P450 24A1 (CYP24A1)

catalog :

MBS2018602

quantity :

0.01 mg

price :

200 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2018602

products type :

Recombinant Protein

products full name :

Recombinant Cytochrome P450 24A1 (CYP24A1)

products short name :

Cytochrome P450 24A1 (CYP24A1)

other names :

1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial; 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial; 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial; 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial; cytochrome P450, 24; cytochrome P450 24A1; cytochrome P450-CC24; cytochrome P450, 24a1; vitamin D(3) 24-hydroxylase; 25-hydroxyvitamin D-24-hydroxylase; cytochrome P450, family 24, subfamily a, polypeptide 1; Cytochrome P450 24A1; Cytochrome P450-CC24

products gene name :

CYP24A1

other gene names :

Cyp24a1; Cyp24a1; CP24; Cyp24; 24-OHase; Cyp-24; Cyp24; 24-OHase

uniprot entry name :

CP24A_MOUSE

host :

E Coli

sequence length :

514

sequence :

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- H KRLNTKVWQA HTLAWDTIFK SVKPCIDHRL ERYSQQPGAD FLCDIYQQDH LSKKELYAAV TELQLAAVET TANSLMWILY NLSRNPQVQQ RLLREIQSVL PDNQTPRAED VRNMPYLKAC LKESMRLTPS VPFTTRTLDK PTVLGEYTLP KGTVLTLNTQ VLGSSEDNFE DADKFRPERW LEKEKKINPF AHLPFGVGKRMCIGRRLAEL QLHLALCWII QKYNIVATDS EPVEMLHLGI LVPSRELPIA FCPR

purity :

> 95%

form :

Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.

storage stability :

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

tested application :

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

other info1 :

Organism: Mus musculus (Mouse). Expression System: Prokaryotic expression. Residues: His260~Arg514 (Accession # Q64441) with two N-terminal Tags, His-tag and T7-tag. Subcellular Location: Mitochondrion

other info2 :

Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Predicted Isoelectric Point: 8.9. Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

products description :

About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.

ncbi gi num :

6753572

ncbi acc num :

NP_034126.1

ncbi gb acc num :

NM_009996.3

uniprot acc num :

Q64441

ncbi mol weight :

33.2kDa

ncbi pathways :

Biological Oxidations Pathway (926856); Cytochrome P450 Pathway (198390); Cytochrome P450 - Arranged By Substrate Type Pathway (926858); Metabolism Pathway (926669); Metabolism Of Lipids And Lipoproteins Pathway (926687); Metabolism Of Steroid Hormones And Vitamin D Pathway (926732); Metapathway Biotransformation (198377); MicroRNAs In Cancer Pathway (852715); MicroRNAs In Cancer Pathway (852928); Nuclear Receptors In Lipid Metabolism And Toxicity Pathway (198356)

ncbi summary :

The protein encoded by this gene localizes to the mitochondrion, where it degrades calcitriol to calcitetrol. This gene is upregulated by binding of calcitriol to the upstream regulatory region and to a downstream enhancer region, thereby allowing calcitriol to autoregulate its concentration in the cell. The encoded protein may also play a role in calcium homeostasis. [provided by RefSeq, Aug 2015]

uniprot summary :

CYP24A1: Has a role in maintaining calcium homeostasis. Catalyzes the NADPH-dependent 24-hydroxylation of calcidiol (25- hydroxyvitamin D(3)) and calcitriol (1-alpha,25-dihydroxyvitamin D(3)). The enzyme can perform up to 6 rounds of hydroxylation of calcitriol leading to calcitroic acid. It also shows 23- hydroxylating activity leading to 1-alpha,25-dihydroxyvitamin D(3)-26,23-lactone as end product. Defects in CYP24A1 are the cause of hypercalcemia infantile (HCAI). HCAI is a disorder characterized by abnormally high level of calcium in the blood, failure to thrive, vomiting, dehydration, and nephrocalcinosis. Belongs to the cytochrome P450 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Oxidoreductase; EC 1.14.13.126; Mitochondrial. Cellular Component: mitochondrion. Molecular Function: oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; iron ion binding; metal ion binding; heme binding; 25-hydroxycholecalciferol-24-hydroxylase activity; oxidoreductase activity; 1-alpha,25-dihydroxyvitamin D3 (1,25-(OH)2D3) 24-hydroxylase activity; monooxygenase activity. Biological Process: response to vitamin D; vitamin D metabolic process

size1 :

0.01 mg

price1 :

200 USD

size2 :

0.05 mg

price2 :

435

size3 :

0.1 mg

price3 :

675

more info or order :

MyBioSource product webpage