Recombinant Glutamate Decarboxylase 2, Acid (GAD2) | MyBioSource MBS2018595 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Glutamate Decarboxylase 2, Acid (GAD2)

catalog :

MBS2018595

quantity :

0.01 mg

price :

200 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2018595

products type :

Recombinant Protein

products full name :

Recombinant Glutamate Decarboxylase 2, Acid (GAD2)

products short name :

Glutamate Decarboxylase 2, Acid (GAD2)

other names :

glutamate decarboxylase 2; Glutamate decarboxylase 2; glutamate decarboxylase 2; GAD-65; 65 kDa glutamic acid decarboxylase; Glutamate decarboxylase-2 (pancreas); glutamate decarboxylase 65 kDa isoform; glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa); 65 kDa glutamic acid decarboxylase; GAD-65; Glutamate decarboxylase 65 kDa isoform

products gene name :

GAD2

other gene names :

GAD2; GAD2; GAD65; GAD65; GAD-65

uniprot entry name :

DCE2_HUMAN

host :

E Coli

sequence length :

585

sequence :

The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below. MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGS- RAAARK AACACDQKPC SCSKVDVNYA FLHATDLLPA CDGER

purity :

> 95%

form :

Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.

storage stability :

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

tested application :

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

other info1 :

Organism: Homo sapiens (Human). Expression System: Prokaryotic expression. Residues: Arg65~Arg105 (Accession # Q05329) with two N-terminal Tags, His-tag and GST-tag

other info2 :

Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4. Predicted isoelectric point: 6.4

products description :

About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.

ncbi gi num :

4503875

ncbi acc num :

NP_000809.1

ncbi gb acc num :

NM_000818.2

uniprot acc num :

Q05329

ncbi mol weight :

36.1kDa

ncbi pathways :

Alanine And Aspartate Metabolism Pathway (198783); Alanine, Aspartate And Glutamate Metabolism Pathway (101142); Alanine, Aspartate And Glutamate Metabolism Pathway (100063); Biogenic Amine Synthesis Pathway (198793); Butanoate Metabolism Pathway (83007); Butanoate Metabolism Pathway (391); GABA (gamma-Aminobutyrate) Shunt Pathway (413433); GABA (gamma-Aminobutyrate) Shunt Pathway (468220); GABA Synthesis Pathway (187175); GABA Synthesis, Release, Reuptake And Degradation Pathway (187174)

ncbi summary :

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2008]

uniprot summary :

GAD2: Catalyzes the production of GABA. Homodimer. Belongs to the group II decarboxylase family. Protein type: Carbohydrate Metabolism - butanoate; EC 4.1.1.15; Lyase; Other Amino Acids Metabolism - taurine and hypotaurine; Other Amino Acids Metabolism - beta-alanine; Amino Acid Metabolism - alanine, aspartate and glutamate. Chromosomal Location of Human Ortholog: 10p11.23. Cellular Component: Golgi membrane; presynaptic membrane; synaptic vesicle membrane; perinuclear region of cytoplasm; axon; plasma membrane; cell junction; cytosol. Molecular Function: protein binding; glutamate binding; glutamate decarboxylase activity; protein heterodimerization activity; pyridoxal phosphate binding. Biological Process: response to drug; synaptic transmission; glutamate decarboxylation to succinate; neurotransmitter secretion; neurotransmitter biosynthetic process

size1 :

0.01 mg

price1 :

200 USD

size2 :

0.05 mg

price2 :

440

size3 :

0.1 mg

price3 :

690

more info or order :

MyBioSource product webpage