protein

Recombinant Cytochrome P450 7A1 (CYP7A1)

MBS2018469

0.01 mg

190 USD

Recombinant Protein

Recombinant Cytochrome P450 7A1 (CYP7A1)

Cytochrome P450 7A1 (CYP7A1)

cholesterol 7-alpha-monooxygenase; Cholesterol 7-alpha-monooxygenase; cholesterol 7-alpha-monooxygenase; CYPVII; cytochrome P450 7A1; cytochrome P450, 7a1; cholesterol 7 alpha hydroxylase; cholesterol 7-alpha-hydroxylase; cytochrome P450, family 7, subfamily a, polypeptide 1; CYPVII; Cholesterol 7-alpha-hydroxylase; Cytochrome P450 7A1

CYP7A1

Cyp7a1; Cyp7a1; Cyp7

CP7A1_MOUSE

E Coli

Organism: Mus musculus (Mouse)

503

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LFKT AHKARERLAE SLKHKNLYMR DQVSELIRLR MFLNDTLSTF DDMEKAKTHL VILWASQANT IPATFWSLFQ MIRSPEAMKA ASEEVNGALQ SAGQELSSGG NAIYLDQEQL NNLPVLDSII KEALRLSSAS LNIRTAKEDF TLHLEDGSYN IRKDDIIALY PQLMHLDPEI YPDPLTFKYD RYLDESGKAKTTFYRNGNKL KYFYMPFGSG ATICPGRLFA VQEIKQFLIL MLSYFEL

> 95%

Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

Expression System: Prokaryotic expression. Residues: Leu227~Leu467 (Accession # Q64505) with two N-terminal Tags, His-tag and T7-tag. Subcellular Location: Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane

Endotoxin Level: less than 1.0EU per 1ug (determined by the LAL method). Predicted Isoelectric Point: 6.9. Reconstitution: Reconstitute in ddH2O

About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.

31542445

NP_031850.2

NM_007824.2

Q64505

31.5kDa

Bile Acid And Bile Salt Metabolism Pathway (926726); Bile Secretion Pathway (193148); Bile Secretion Pathway (193095); Biological Oxidations Pathway (926856); Cytochrome P450 Pathway (198390); Cytochrome P450 - Arranged By Substrate Type Pathway (926858); Endogenous Sterols Pathway (926859); Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway (926696); Metabolism Pathway (926669); Metabolism Of Lipids And Lipoproteins Pathway (926687)

CYP7A1: Catalyzes a rate-limiting step in cholesterol catabolism and bile acid biosynthesis by introducing a hydrophilic moiety at position 7 of cholesterol. Important for cholesterol homeostasis. Belongs to the cytochrome P450 family. Protein type: EC 1.14.13.17; Lipid Metabolism - primary bile acid biosynthesis; Endoplasmic reticulum; Oxidoreductase. Cellular Component: intracellular membrane-bound organelle; membrane; endoplasmic reticulum. Molecular Function: oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; cholesterol 7-alpha-monooxygenase activity; metal ion binding; iron ion binding; heme binding; oxidoreductase activity; monooxygenase activity. Biological Process: steroid metabolic process; cholesterol metabolic process; bile acid biosynthetic process; cholesterol homeostasis; lipid metabolic process; cholesterol catabolic process

0.01 mg

190 USD

0.05 mg

410

0.1 mg

635