catalog number :
MBS2018433
products type :
Recombinant Protein
products full name :
Recombinant Galectin 12 (GAL12)
products short name :
Galectin 12 (GAL12)
other names :
galectin-12 isoform 1; Galectin-12; galectin-12; galectin 12; galectin-related inhibitor of proliferation; lectin, galactoside-binding, soluble, 12; Galectin-related inhibitor of proliferation
products gene name :
GAL12
other gene names :
LGALS12; LGALS12; GAL12; GRIP1; GRIP1; Gal-12
uniprot entry name :
LEG12_HUMAN
reactivity :
Homo sapiens (Human)
sequence :
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- P LDAHRFQVDF QCGCSLCPRP DIAFHFNPRF HTTKPHVICN TLHGGRWQRE ARWPHLALRR GSSFLILFLF GNEEVKVSVN GQHFLHFRYR LPLSHVDTLG IFGDILVEAV GFLNINPFVE GSREYPAGHP FLLMSPRLEV PCSHALPQGL SPGQVIIVRG LVLQEPKHFT VSLRDQAAHA PVTLRASFADRTLAWISRWG QKKLISAPFL FYPQRFFEVL L
form :
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarkosyl, 5%Trehalose and Proclin300.
storage stability :
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
tested application :
SDS-PAGE; WB; ELISA; IP; CoIP; ReporterAssays; Purification; Amine Reactive Labeling. (May be suitable for use in other assays to be determined by the end user.)
other info1 :
Tags: N-terminal His-Tag. Tissue Specificity: Blood. Expression System: Prokaryotic expression. Residues: Pro70~Leu291 (Accession # Q96DT0) with two N-terminal Tags, His-tag and T7-tag. Subcellular Location: Nucleus. Traits: Freeze-dried powder
other info2 :
Predicted isoelectric point: 8.7. Predicted Molecular Mass: 29.0kDa. Accurate Molecular Mass: 27kDa as determined by SDS-PAGE reducing conditions. Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
ncbi acc num :
NP_001136007.1
ncbi gb acc num :
NM_001142535.1
ncbi summary :
This gene encodes a member of the galectin superfamily, a group of beta-galactoside-binding proteins with conserved carbohydrate recognition domains. The related mouse protein is a primary regulator of the early stages of adipose tissue development. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, May 2010]
uniprot summary :
LGALS12: Binds lactose. May participate in the apoptosis of adipocytes. 6 isoforms of the human protein are produced by alternative splicing. Chromosomal Location of Human Ortholog: 11q13. Cellular Component: mitochondrion; nucleus. Molecular Function: lactose binding