protein

Recombinant Nuclear Receptor Subfamily 3, Group C, Member 1 (NR3C1)

MBS2018413

0.01 mg

185 USD

Recombinant Protein

Recombinant Nuclear Receptor Subfamily 3, Group C, Member 1 (NR3C1)

Nuclear Receptor Subfamily 3, Group C, Member 1 (NR3C1)

glucocorticoid receptor isoform alpha; Glucocorticoid receptor; glucocorticoid receptor; glucocorticoid nuclear receptor variant 1; nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor); Nuclear receptor subfamily 3 group C member 1

NR3C1

NR3C1; NR3C1; GR; GCR; GRL; GCCR; GRL; GR

GCR_HUMAN

E Coli

Homo sapiens (Human)

777

The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below. MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-LVSLLEVIE PEVLYAGYDS SVPDSTWRIM TTLNMLGGRQ VIAAVKWAKA IPGFRNLHLD DQMTLLQYSW MFLMAFALGW RSYRQSSANL LCFAPDLIIN EQRMTLPCMY DQCKHMLYVS SELHRLQVSY EEYLCMKTLL LLSSVPKDGL KSQELFDEIR MTYIKELGKA IVKREGNSSQ NWQRFYQLTKLLDSMHEVVE

> 95%

Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

Subcellular Location: Cytoplasm. Mitochondrion. Nucleus. Expression System: Prokaryotic expression. Residues: Leu532~Glu730 (Accession # P04150) with two N-terminal Tags, His-tag and GST-tag. Subcellular Location: Cytoplasm. Mitochondrion. Nucleus

Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Predicted Isoelectric Point: 6.3. Reconstitution: Reconstitute in ddH2O.

About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.

4504133

NP_000167.1

NM_000176.2

P04150

55.6kDa

Adipogenesis Pathway (198832); BMAL1:CLOCK/NPAS2 Activates Circadian Expression Pathway (477138); Circadian Clock Pathway (187173); FOXA2 And FOXA3 Transcription Factor Networks Pathway (137911); Gene Expression Pathway (105937); Generic Transcription Pathway (105938); Glucocorticoid Receptor Regulatory Network Pathway (138014); Neuroactive Ligand-receptor Interaction Pathway (83053); Neuroactive Ligand-receptor Interaction Pathway (462); Nuclear Receptor Transcription Pathway (105979)

This gene encodes glucocorticoid receptor, which can function both as a transcription factor that binds to glucocorticoid response elements in the promoters of glucocorticoid responsive genes to activate their transcription, and as a regulator of other transcription factors. This receptor is typically found in the cytoplasm, but upon ligand binding, is transported into the nucleus. It is involved in inflammatory responses, cellular proliferation, and differentiation in target tissues. Mutations in this gene are associated with generalized glucocorticoid resistance. Alternative splicing of this gene results in transcript variants encoding either the same or different isoforms. Additional isoforms resulting from the use of alternate in-frame translation initiation sites have also been described, and shown to be functional, displaying diverse cytoplasm-to-nucleus trafficking patterns and distinct transcriptional activities (PMID:15866175). [provided by RefSeq, Feb 2011]

GR: transcription factor of the nuclear receptor family. Receptor for glucocorticoids (GC). Binds to glucocorticoid response elements (GRE) and modulates other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Three alternatively spliced isoforms have been described. Protein type: Nuclear receptor; Transcription factor; DNA-binding; Mitochondrial. Chromosomal Location of Human Ortholog: 5q31.3. Cellular Component: nucleoplasm; membrane; mitochondrial matrix; cytoplasm; cytosol; nucleus. Molecular Function: protein dimerization activity; glucocorticoid receptor activity; protein binding; zinc ion binding; transcription factor activity; steroid binding. Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; adrenal gland development; maternal behavior; regulation of glucocorticoid biosynthetic process; chromatin modification; signal transduction; glucocorticoid mediated signaling; glucocorticoid metabolic process; regulation of transcription, DNA-dependent; positive regulation of neuron apoptosis; glucocorticoid receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; regulation of gluconeogenesis; gene expression. Disease: Glucocorticoid Resistance, Generalized

0.01 mg

185 USD

0.05 mg

395

0.1 mg

605