protein

Recombinant Caspase Activated DNase (CAD)

MBS2018293

0.01 mg

145 USD

Recombinant Protein

Recombinant Caspase Activated DNase (CAD)

[Caspase Activated DNase (CAD)]

[DNA fragmentation factor subunit beta isoform 2; DNA fragmentation factor subunit beta; DNA fragmentation factor subunit beta; caspase-activated DNase; caspase-activated nuclease; caspase-activated deoxyribonuclease; DNA fragmentation factor 40 kDa subunit; DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase); Caspase-activated deoxyribonuclease; CAD; Caspase-activated DNase; Caspase-activated nuclease; CPAN; DNA fragmentation factor 40 kDa subunit]

[CAD]

[DFFB; DFFB; CAD; CPAN; DFF2; DFF40; DFF-40; CAD; DFF2; DFF40; CAD; Caspase-activated DNase; CPAN; DFF-40]

DFFB_HUMAN

E.coli

> 95%

Original Concentration: 200 ug/mL

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8ºC for one month. Aliquot and store at -80ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Positive Control; Immunogen; SDS-PAGE; WB. (May be suitable for use in other assays to be determined by the end user.)

SDS-Page

Organism Species: Homo sapiens(Human). Source: Prokaryotic expression. Residues: Arg87~Lys323. Tags: N-terminal His Tag. Subcellular Location: Nucleus, Cytoplasm. Traits: Freeze-dried powder. Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.

Predicted isoelectric point: 8.4. Predicted Molecular Mass: 31.1kDa. Accurate Molecular Mass: 29kDa as determined by SDS-PAGE reducing conditions. Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

4758150

NP_004393.1

NM_004402.3

O76075

Activation Of DNA Fragmentation Factor Pathway (105683); Apoptosis Pathway (198797); Apoptosis Pathway (83060); Apoptosis Pathway (470); Apoptosis Pathway (105648); Apoptosis Modulation And Signaling Pathway (198822); Apoptosis Induced DNA Fragmentation Pathway (105682); Apoptotic Execution Phase Pathway (105677); Caspase Cascade In Apoptosis Pathway (137974); FAS Pathway And Stress Induction Of HSP Regulation (198894)

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of some of these variants has not been determined. [provided by RefSeq, Sep 2013]

DFFB: Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Deoxyribonuclease; Apoptosis; EC 3.-.-.-. Chromosomal Location of Human Ortholog: 1p36.3. Cellular Component: nucleoplasm; nuclear chromatin; nucleolus; nucleus; cytosol. Molecular Function: enzyme binding; deoxyribonuclease activity. Biological Process: apoptosis; DNA fragmentation during apoptosis; apoptotic chromosome condensation; cell structure disassembly during apoptosis

0.01 mg

145 USD

0.05 mg

285

0.1 mg

445

0.2 mg

545

0.5 mg

1055

1 mg

1575