Recombinant Cystathionine Gamma Lyase (CSE) | MyBioSource MBS2018289 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Cystathionine Gamma Lyase (CSE)

catalog :

MBS2018289

quantity :

0.01 mg

price :

150 USD

more info or order :

MyBioSource product webpage

image

image 1 :

image 2 :

product information

catalog number :

MBS2018289

products type :

Recombinant Protein

products full name :

Recombinant Cystathionine Gamma Lyase (CSE)

products short name :

[Cystathionine Gamma Lyase (CSE)]

other names :

[cystathionine gamma-lyase isoform 3; Cystathionine gamma-lyase; cystathionine gamma-lyase; gamma-cystathionase; homoserine deaminase; cysteine desulfhydrase; homoserine dehydratase; cysteine-protein sulfhydrase; cystathionase (cystathionine gamma-lyase); Cysteine-protein sulfhydrase; Gamma-cystathionase]

products gene name :

[CSE]

other gene names :

[CTH; CTH]

uniprot entry name :

CGL_HUMAN

host :

E. coli

purity :

>90%

form :

20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300

concentration :

200ug/mL

storage stability :

Storage: . Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months. Stability Test :. The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

tested application :

Positive Control, Immunogen, SDS-PAGE, Western Blot (WB). (May be suitable for use in other assays to be determined by the end user.)

image1 heading :

Sequence Information

image2 heading :

SDS-Page

other info1 :

Organism Species: Homo sapiens (Human). Source: Prokaryotic expression. Residues: Thr163~Asp382. Tags: N-terminal His-Tag. Subcellular Location: Cytoplasm. Traits: Freeze-dried powder

other info2 :

Predicted isoelectric point: 7.3. Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

ncbi gi num :

299473759

ncbi acc num :

NP_001177392.1

ncbi gb acc num :

NM_001190463.1

uniprot acc num :

P32929

ncbi mol weight :

Predicted Molecular Mass: 28.1kDa. Accurate Molecular Mass: 26kDa as determined by SDS-PAGE reducing conditions

ncbi pathways :

Biosynthesis Of Amino Acids Pathway (790012); Biosynthesis Of Amino Acids Pathway (795174); Cysteine And Methionine Metabolism Pathway (104488); Cysteine And Methionine Metabolism Pathway (103421); Cysteine Biosynthesis, Homocysteine + Serine = Cysteine Pathway (413414); Cysteine Biosynthesis, Homocysteine + Serine = Cysteine Pathway (468303); Cysteine Formation From Homocysteine Pathway (530754); Degradation Of Cysteine And Homocysteine Pathway (530755); Glycine, Serine And Threonine Metabolism Pathway (82949); Glycine, Serine And Threonine Metabolism Pathway (313)

ncbi summary :

This gene encodes a cytoplasmic enzyme in the trans-sulfuration pathway that converts cystathione derived from methionine into cysteine. Glutathione synthesis in the liver is dependent upon the availability of cysteine. Mutations in this gene cause cystathioninuria. Alternative splicing of this gene results in three transcript variants encoding different isoforms. [provided by RefSeq, Jun 2010]

uniprot summary :

CTH: Catalyzes the last step in the trans-sulfuration pathway from methionine to cysteine. Has broad substrate specificity. Converts cystathionine to cysteine, ammonia and 2-oxobutanoate. Converts two cysteine molecules to lanthionine and hydrogen sulfide. Can also accept homocysteine as substrate. Specificity depends on the levels of the endogenous substrates. Generates the endogenous signaling molecule hydrogen sulfide (H2S), and so contributes to the regulation of blood pressure. Acts as a cysteine-protein sulfhydrase by mediating sulfhydration of target proteins: sulfhydration consists of converting -SH groups into -SSH on specific cysteine residues of target proteins such as GAPDH, PTPN1 and NF-kappa-B subunit RELA, thereby regulating their function. Defects in CTH are the cause of cystathioninuria (CSTNU). It is an autosomal recessive phenotype characterized by abnormal accumulation of plasma cystathionine, leading to increased urinary excretion. Belongs to the trans-sulfuration enzymes family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Amino Acid Metabolism - glycine, serine and threonine; Cell cycle regulation; EC 4.4.1.1; Energy Metabolism - nitrogen; Other Amino Acids Metabolism - selenoamino acid; Amino Acid Metabolism - cysteine and methionine; Lyase. Chromosomal Location of Human Ortholog: 1p31.1. Cellular Component: nucleoplasm; cytoplasm; nucleus; cytosol. Molecular Function: calmodulin binding; protein binding; cystathionine gamma-lyase activity; homocysteine desulfhydrase activity; carbon-sulfur lyase activity; pyridoxal phosphate binding. Biological Process: cysteine metabolic process; positive regulation of I-kappaB kinase/NF-kappaB cascade; unfolded protein response; sulfur amino acid metabolic process; protein-pyridoxal-5-phosphate linkage via peptidyl-N6-pyridoxal phosphate-L-lysine; sulfur amino acid catabolic process; transsulfuration; cysteine biosynthetic process; protein homotetramerization; activation of NF-kappaB transcription factor. Disease: Cystathioninuria

size1 :

0.01 mg

price1 :

150 USD

size2 :

0.05 mg

price2 :

285

size3 :

0.1 mg

price3 :

445

size4 :

0.2 mg

price4 :

550

size5 :

0.5 mg

price5 :

1060

size6 :

1 mg

price6 :

1580

more info or order :

MyBioSource product webpage