FITC-linked Antibody to Tissue Factor (TF) | MyBioSource MBS2017563 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

FITC-linked Antibody to Tissue Factor (TF)

catalog :

MBS2017563

quantity :

0.1 mg

price :

395 USD

clonality :

polyclonal

host :

rabbit

conjugate :

FITC

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2017563

products type :

Antibody

products full name :

FITC-linked Antibody to Tissue Factor (TF)

products short name :

Tissue Factor (TF)

other names :

tissue factor; Tissue factor; tissue factor; coagulation factor III (thromboplastin, tissue factor); Coagulation factor III; Thromboplastin

products gene name :

other gene names :

F3; F3; TF; TFA; CD142; TF

uniprot entry name :

TF_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Mouse

sequence length :

295

specificity :

The antibody is a rabbit polyclonal antibody raised against TF. It has been selected for its ability to recognize TF in immunohistochemical staining and western blotting.

purity :

Affinity Chromatography

form :

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.

concentration :

200ug/ml

storage stability :

Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

tested application :

Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB), ELISA

app notes :

Western blotting: 1:50-400. Immunocytochemistry in formalin fixed cells: 1:50-500. Immunohistochemistry in formalin fixed frozen section: 1:50-500. Immunohistochemistry in paraffin section: 1:10-100. Enzyme-linked Immunosorbent Assay: 1:100-200. Optimal working dilutions must be determined by end user.

other info1 :

Immunogen: Recombinant TF (Gly30~Glu251) N-terminal His-Tag expressed in E.coli. Accession No.: RPA524Mu01

other info2 :

Conjugation: FITC. Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

products categories :

FITC Antibody

ncbi gi num :

339508

ncbi acc num :

AAA36734.1

uniprot acc num :

P13726

ncbi pathways :

Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Extrinsic Pathway (106058); Formation Of Fibrin Clot (Clotting Cascade) Pathway (106057); Hemostasis Pathway (106028)

ncbi summary :

This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

uniprot summary :

F3: Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade. Interacts with HSPE; the interaction, inhibited by heparin, promotes the generation of activated factor X and activates coagulation in the presence of activated factor VII. TF expression is highly dependent upon cell type. TF can also be induced by the inflammatory mediators interleukin 1 and TNF-alpha, as well as by endotoxin, to appear on monocytes and vascular endothelial cells as a component of cellular immune response. Lung, placenta and pancreas. Belongs to the tissue factor family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Cell surface; Membrane protein, integral. Chromosomal Location of Human Ortholog: 1p22-p21. Cellular Component: extracellular matrix; extracellular space; cell surface; integral to membrane; plasma membrane. Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein binding; protease binding; phospholipid binding. Biological Process: caspase activation; activation of blood coagulation via clotting cascade; positive regulation of protein kinase B signaling cascade; blood coagulation, extrinsic pathway; positive regulation of angiogenesis; activation of plasma proteins during acute inflammatory response; positive regulation of positive chemotaxis; cytokine and chemokine mediated signaling pathway; positive regulation of endothelial cell proliferation; blood coagulation; positive regulation of cell migration

size1 :

0.1 mg

price1 :

395 USD

more info or order :

MyBioSource product webpage