Polyclonal Antibody to Fucosidase Alpha L1, Tissue (FUCa1) | MyBioSource MBS2016213 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Polyclonal Antibody to Fucosidase Alpha L1, Tissue (FUCa1)

catalog :

MBS2016213

quantity :

0.01 mg

price :

105 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot: Sample: Lane1: Rat Liver Tissue; Lane2: Mouse Liver Tissue.

image 2 :

Western Blot: Sample: Recombinant FUCa1, Mouse.

image 3 :

DAB staining on fromalin fixed paraffin-embedded Kidney tissue)

product information

catalog number :

MBS2016213

products type :

Antibody

products full name :

Polyclonal Antibody to Fucosidase Alpha L1, Tissue (FUCa1)

products short name :

[Fucosidase Alpha L1, Tissue (FUCa1)]

other names :

[tissue alpha-L-fucosidase; Tissue alpha-L-fucosidase; tissue alpha-L-fucosidase; fucosidase, alpha-L- 1, tissue; Alpha-L-fucosidase I; Alpha-L-fucoside fucohydrolase 1; Alpha-L-fucosidase 1]

products gene name :

[FUCa1]

other gene names :

[Fuca1; Fuca1; Afuc; Fuca; 0610006A03Rik; 9530055J05Rik; Fuca; Alpha-L-fucosidase 1]

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Mouse

sequence length :

451

sequence :

Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- NGFKTQ HFVRAKTMPE LYDLVNSYKP DLIWSDGEWE CPDTYWNSTS FLAWLYNDSP VKDEVIVNDR WGQNCSCHHG GYYNCQDKYK PQSLPDHKWE MCTSMDRASW GYRKDMTMST IAKENEIIEE LVQTVSLGGN YLLNIGPTKD GLIVPIFQER LLAVGKWLQI NGEAIYASKP WRVQSEKNKT VVWYTTKNAT VYATFLYWPE NGIVNLKSPK TTSATKITML GLEGDLSWTQ

specificity :

The antibody is a rabbit polyclonal antibody raised against FUCa1. It has been selected for its ability to recognize FUCa1 in immunohistochemical staining andwestern blotting.

purity :

Affinity Chromatography

form :

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.

concentration :

200ug/ml

storage stability :

Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

tested application :

Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)

app notes :

Western blotting: 1:100-400. Immunocytochemistry in formalin fixed cells: 1:100-500. Immunohistochemistry in formalin fixed frozen section: 1:100-500. Immunohistochemistry in paraffin section: 1:50-200. Enzyme-linked Immunosorbent Assay: 1:100-200

image1 heading :

Western Blot (WB)

image2 heading :

Western Blot (WB)

image3 heading :

Immunohistochemistry (IHC)

other info1 :

Immunogen: FUCa1, Mouse. Immunogen information: Recombinant FUCa1 (Asn185~Lys447) with two N-terminal Tags, His-tag and T7-tag expressed in E.coli. Accession No.: RPA807Mu01

other info2 :

Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2047774

ncbi gi num :

169808427

ncbi acc num :

NP_077205.3

ncbi gb acc num :

NM_024243.4

uniprot acc num :

Q99LJ1

ncbi mol weight :

52,281 Da

ncbi pathways :

Lysosome Pathway (99272); Lysosome Pathway (96865); Other Glycan Degradation Pathway (83175); Other Glycan Degradation Pathway (346)

uniprot summary :

FUCA1: Alpha-L-fucosidase is responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N- acetylglucosamine of the carbohydrate moieties of glycoproteins. Defects in FUCA1 are the cause of fucosidosis (FUCA1D). FUCA1D is an autosomal recessive lysosomal storage disease characterized by accumulation of fucose-containing glycolipids and glycoproteins in various tissues. Clinical signs include facial dysmorphism, dysostosis multiplex, moderate hepatomegaly, severe intellectual deficit, deafness, and according to age, angiokeratomas. Belongs to the glycosyl hydrolase 29 family. Protein type: EC 3.2.1.51; Glycan Metabolism - other glycan degradation; Hydrolase. Chromosomal Location of Human Ortholog: 4 D3 4 68.01 cM. Cellular Component: lysosome. Molecular Function: alpha-L-fucosidase activity; carbohydrate binding; fucosidase activity; hydrolase activity; hydrolase activity, acting on glycosyl bonds. Biological Process: carbohydrate metabolic process; fucose metabolic process; glycoside catabolic process; metabolic process

size1 :

0.01 mg

price1 :

105 USD

size2 :

0.02 mg

price2 :

125

size3 :

0.05 mg

price3 :

170

size4 :

0.1 mg

price4 :

210

size5 :

0.2 mg

price5 :

315

size6 :

1 mg

price6 :

745

more info or order :

MyBioSource product webpage