catalog number :
MBS2016164
products full name :
Polyclonal Antibody to Spondin 1 (SPON1)
products short name :
[Spondin 1 (SPON1)]
other names :
[spondin-1; Spondin-1; spondin-1; spondin 1; F-spondin; Vascular smooth muscle cell growth-promoting factor]
products gene name :
[SPON1]
other gene names :
[SPON1; SPON1; f-spondin; VSGP/F-spondin; KIAA0762; VSGP]
reactivity :
Homo sapiens (Human)
sequence :
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG S- AETS QAEKCMMPEC HTIPCLLSPW SEWSDCSVTC GKGMRTRQRM LKSLAELGDC NEDLEQVEKC MLPECPIDCE LTEWSQWSEC NKSCGKGHVI RTRMIQMEPQ FGGAPCPETV QRKKCRIRKC LRNPSIQKLR WREARESRRS EQLKEESEGE
specificity :
The antibody is a rabbit polyclonal antibody raised against SPON1. It has been selected for its ability to recognize SPON1 in immunohistochemical staining andwestern blotting.
purity :
Affinity Chromatography
form :
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
storage stability :
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
tested application :
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
app notes :
Western blotting: 1:50-400. Immunocytochemistry in formalin fixed cells: 1:50-500. Immunohistochemistry in formalin fixed frozen section: 1:50-500. Immunohistochemistry in paraffin section: 1:10-100. Enzyme-linked Immunosorbent Assay: 1:100-5000. Optimal working dilutions must be determined by end user.
image1 heading :
Western Blot (WB)
image2 heading :
Western Blot (WB)
image3 heading :
Immunohistochemistry (IHC)
other info1 :
Immunogen: SPON1, Human. Immunogen Information: Recombinant SPON1 (Ala597~Ala801) with N-terminal His-Tag
expressed in E.coli. Accession No.: MBS2016164
other info2 :
Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2076486
ncbi acc num :
NP_006099.2
ncbi gb acc num :
NM_006108.3
ncbi mol weight :
90,973 Da
ncbi pathways :
Metabolism Of Proteins Pathway (106230); O-glycosylation Of TSR Domain-containing Proteins Pathway (980460); O-linked Glycosylation Pathway (980459); Post-translational Protein Modification Pathway (161012)
uniprot summary :
SPON1: Cell adhesion protein that promotes the attachment of spinal cord and sensory neuron cells and the outgrowth of neurites in vitro. May contribute to the growth and guidance of axons in both the spinal cord and the PNS. Major factor for vascular smooth muscle cell. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 11p15.2. Cellular Component: endoplasmic reticulum lumen; extracellular matrix; extracellular space; proteinaceous extracellular matrix. Molecular Function: metal ion binding; protein binding. Biological Process: cell adhesion