Biotin-Linked Antibody to Cyclic ADP Ribose Hydrolase (cADPRH) | MyBioSource MBS2015818 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Biotin-Linked Antibody to Cyclic ADP Ribose Hydrolase (cADPRH)

catalog :

MBS2015818

quantity :

0.1 mg

price :

365 USD

clonality :

polyclonal

host :

rabbit

conjugate :

biotin

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2015818

products type :

Antibody

products full name :

Biotin-Linked Antibody to Cyclic ADP Ribose Hydrolase (cADPRH)

products short name :

Cyclic ADP Ribose Hydrolase (cADPRH)

other names :

cyclic ADP-ribose hydrolase; ADP-ribosyl cyclase 1; ADP-ribosyl cyclase 1; cADPr hydrolase 1; CD38 antigen (p45); NAD(+) nucleosidase; cyclic ADP-ribose hydrolase 1; ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase; CD38 molecule; Cyclic ADP-ribose hydrolase 1; cADPr hydrolase 1; T10

products gene name :

cADPRH

other gene names :

CD38; CD38; T10; cADPr hydrolase 1

uniprot entry name :

CD38_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Mouse

sequence length :

specificity :

The antibody is a rabbit polyclonal antibody raised against cADPRH. It has been selected for its ability to recognize cADPRH in immunohistochemical staining and western blotting.

purity :

Affinity Chromatography

form :

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

concentration :

200ug/ml

storage stability :

Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

tested application :

Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)

app notes :

Western blotting: 1:50-400. Immunocytochemistry in formalin fixed cells: 1:50-500. Immunohistochemistry in formalin fixed frozen section: 1:50-500. Immunohistochemistry in paraffin section: 1:10-100. Enzyme-linked Immunosorbent Assay: 1:100-200. Optimal working dilutions must be determined by end user.

other info1 :

Immunogen: cADPRH, Mouse. Conjugation: Biotin. Immunogen: Recombinant cADPRH (Thr54~Arg301) with two N-terminal Tags, His-tag and T7-tag expressed in E.coli. Accession No.: RPA752Mu01

other info2 :

Quality Control: Content: The quality control contains recombinant cADPRH (Thr54~Arg301) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the sbstrate. 5uL per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.

products categories :

Biotin Antibody

ncbi gi num :

68989438

ncbi acc num :

AAY99634.1

uniprot acc num :

P28907

ncbi mol weight :

34,328 Da

ncbi pathways :

Calcium Signaling Pathway (83050); Calcium Signaling Pathway (459); Epstein-Barr Virus Infection Pathway (585562); Epstein-Barr Virus Infection Pathway (587115); Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489); Nicotinate And Nicotinamide Metabolism Pathway (83014); Nicotinate And Nicotinamide Metabolism Pathway (400); Pancreatic Secretion Pathway (169306); Pancreatic Secretion Pathway (169295)

ncbi summary :

The protein encoded by this gene is a non-lineage-restricted, type II transmembrane glycoprotein that synthesizes and hydrolyzes cyclic adenosine 5'-diphosphate-ribose, an intracellular calcium ion mobilizing messenger. The release of soluble protein and the ability of membrane-bound protein to become internalized indicate both extracellular and intracellular functions for the protein. This protein has an N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites. Crystal structure analysis demonstrates that the functional molecule is a dimer, with the central portion containing the catalytic site. It is used as a prognostic marker for patients with chronic lymphocytic leukemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

uniprot summary :

CD38: Synthesizes cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system. Belongs to the ADP-ribosyl cyclase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; EC 2.4.99.20; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Hydrolase; Apoptosis; Cell surface; Cell cycle regulation; EC 3.2.2.6; Lyase. Chromosomal Location of Human Ortholog: 4p15. Cellular Component: cell surface; membrane; integral to membrane; plasma membrane; nucleus. Molecular Function: transferase activity; phosphorus-oxygen lyase activity; NAD+ nucleosidase activity; NAD(P)+ nucleosidase activity. Biological Process: response to drug; response to retinoic acid; metabolic process; positive regulation of transcription, DNA-dependent; positive regulation of insulin secretion; female pregnancy; signal transduction; positive regulation of cell growth; response to estradiol stimulus; B cell receptor signaling pathway; elevation of cytosolic calcium ion concentration; response to hydroperoxide; positive regulation of vasoconstriction; response to hypoxia; positive regulation of B cell proliferation; negative regulation of bone resorption; negative regulation of transcription, DNA-dependent; response to progesterone stimulus; negative regulation of apoptosis

size1 :

0.1 mg

price1 :

365 USD

more info or order :

MyBioSource product webpage