Polyclonal Antibody to Cystathionine Gamma Lyase (CSE) | MyBioSource MBS2014844 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Polyclonal Antibody to Cystathionine Gamma Lyase (CSE)

catalog :

MBS2014844

quantity :

0.01 mg

price :

105 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot: Sample: Porcine Liver Tissue.

image 2 :

Western Blot: Sample: Recombinant CSE, Human.

image 3 :

DAB staining on IHC-P; Samples: Human Kidney Tissue

product information

catalog number :

MBS2014844

products type :

Antibody

products full name :

Polyclonal Antibody to Cystathionine Gamma Lyase (CSE)

products short name :

[Cystathionine Gamma Lyase (CSE)]

other names :

[cystathionine gamma-lyase isoform 3; Cystathionine gamma-lyase; cystathionine gamma-lyase; cystathionine gamma-lyase; Cysteine-protein sulfhydrase; Gamma-cystathionase]

products gene name :

[CSE]

other gene names :

[CTH; CTH]

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

purity :

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

form :

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.

concentration :

0.6mg/ml

storage stability :

Avoid repeated freeze/thaw cycles. Store at 4ºC for frequent use. Aliquot and store at -20ºC for 12 months. The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

tested application :

Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blot (WB), Immunoprecipitation (IP)

app notes :

Western blotting: 0.5-2ug/mL;1:300-1200. Immunohistochemistry: 5-20ug/mL;1:30-120. Immunocytochemistry: 5-20ug/mL;1:30-120. Optimal working dilutions must be determined by end user.

image1 heading :

Western Blot (WB)

image2 heading :

Western Blot (WB)

image3 heading :

Immunohistochemistry (IHC)

image4 heading :

Immunohistochemistry (IHC)

image4 description :

DAB staining on IHC-P; Samples: Human Liver Tissue.

other info1 :

Organism Species: Homo sapiens (Human). Source: Polyclonal antibody preparation. Traits: Liquid. Immunogen: Recombinant CTH (Thr163~Asp382) expressed in E.coli

other info2 :

Western blotting: 0.5-2 ug/mL;1:300-1200. Immunohistochemistry: 5-20 ug/mL;1:30-120. Immunocytochemistry: 5-20 ug/mL;1:30-120. Optimal working dilutions must be determined by end user.

ncbi gi num :

299473759

ncbi acc num :

NP_001177392.1

ncbi gb acc num :

NM_001190463.1

uniprot acc num :

P32929

ncbi pathways :

Biosynthesis Of Amino Acids Pathway (790012); Biosynthesis Of Amino Acids Pathway (795174); Cysteine And Methionine Metabolism Pathway (104488); Cysteine And Methionine Metabolism Pathway (103421); Cysteine Biosynthesis, Homocysteine + Serine = Cysteine Pathway (413414); Cysteine Biosynthesis, Homocysteine + Serine = Cysteine Pathway (468303); Cysteine Formation From Homocysteine Pathway (530754); Degradation Of Cysteine And Homocysteine Pathway (530755); Glycine, Serine And Threonine Metabolism Pathway (82949); Glycine, Serine And Threonine Metabolism Pathway (313)

ncbi summary :

This gene encodes a cytoplasmic enzyme in the trans-sulfuration pathway that converts cystathione derived from methionine into cysteine. Glutathione synthesis in the liver is dependent upon the availability of cysteine. Mutations in this gene cause cystathioninuria. Alternative splicing of this gene results in three transcript variants encoding different isoforms. [provided by RefSeq, Jun 2010]

uniprot summary :

CTH: Catalyzes the last step in the trans-sulfuration pathway from methionine to cysteine. Has broad substrate specificity. Converts cystathionine to cysteine, ammonia and 2-oxobutanoate. Converts two cysteine molecules to lanthionine and hydrogen sulfide. Can also accept homocysteine as substrate. Specificity depends on the levels of the endogenous substrates. Generates the endogenous signaling molecule hydrogen sulfide (H2S), and so contributes to the regulation of blood pressure. Acts as a cysteine-protein sulfhydrase by mediating sulfhydration of target proteins: sulfhydration consists of converting -SH groups into -SSH on specific cysteine residues of target proteins such as GAPDH, PTPN1 and NF-kappa-B subunit RELA, thereby regulating their function. Defects in CTH are the cause of cystathioninuria (CSTNU). It is an autosomal recessive phenotype characterized by abnormal accumulation of plasma cystathionine, leading to increased urinary excretion. Belongs to the trans-sulfuration enzymes family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Amino Acid Metabolism - cysteine and methionine; Amino Acid Metabolism - glycine, serine and threonine; Cell cycle regulation; EC 4.4.1.1; Energy Metabolism - nitrogen; Lyase; Other Amino Acids Metabolism - selenoamino acid. Chromosomal Location of Human Ortholog: 1p31.1. Cellular Component: cytoplasm; cytosol. Molecular Function: calmodulin binding; carbon-sulfur lyase activity; cystathionine gamma-lyase activity; cystathionine gamma-synthase activity; homocysteine desulfhydrase activity; identical protein binding; protein binding; pyridoxal phosphate binding. Biological Process: cysteine biosynthetic process; cysteine biosynthetic process via cystathionine; cysteine metabolic process; endoplasmic reticulum unfolded protein response; positive regulation of I-kappaB kinase/NF-kappaB signaling; positive regulation of NF-kappaB transcription factor activity; protein homotetramerization; protein-pyridoxal-5-phosphate linkage via peptidyl-N6-pyridoxal phosphate-L-lysine; sulfur amino acid catabolic process; transsulfuration. Disease: Cystathioninuria

size1 :

0.01 mg

price1 :

105 USD

size2 :

0.02 mg

price2 :

125

size3 :

0.05 mg

price3 :

170

size4 :

0.1 mg

price4 :

220

size5 :

0.2 mg

price5 :

320

size6 :

1 mg

price6 :

760

more info or order :

MyBioSource product webpage