FITC-linked Antibody to Cubilin (CUBN) | MyBioSource MBS2014578 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

FITC-linked Antibody to Cubilin (CUBN)

catalog :

MBS2014578

quantity :

0.1 mg

price :

265 USD

clonality :

polyclonal

host :

rabbit

conjugate :

FITC

reactivity :

mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Figure1. Western Blot. A. Sample: Recombinant CUBN, Mouse

product information

catalog number :

MBS2014578

products type :

Antibody

products full name :

FITC-linked Antibody to Cubilin (CUBN)

products short name :

[Cubilin (CUBN)]

other names :

[cubilin; Cubilin; cubilin; 460 kDa receptor; glycoprotein 280; intrinsic factor-cobalamin receptor; intrinsic factor-vitamin B12 receptor; cubilin (intrinsic factor-cobalamin receptor); 460 kDa receptor; Glycoprotein 280; gp280; Intrinsic factor-cobalamin receptor; Intrinsic factor-vitamin B12 receptor]

products gene name :

[CUBN]

other gene names :

[Cubn; Cubn; IFCR; MGA1; GP280; Ifcr; gp280]

uniprot entry name :

CUBN_RAT

clonality :

Polyclonal

host :

Rabbit

reactivity :

Mouse

sequence length :

3623

purity :

Antigen-specific Affinity Chromatography.

form :

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

concentration :

200ug/ml

storage stability :

Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition.

tested application :

Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB), ELISA

app notes :

Western blotting: 0.5-2ug/ml. Immunocytochemistry in formalin fixed cells: 5-20ug/ml. Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml. Immunohistochemistry in paraffin section: 5-20ug/ml. Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml. Optimal working dilutions must be determined by end user.

image1 heading :

Western Blot

other info1 :

Source: Antibody labeling

other info2 :

Label: FITC. Traits: Liquid. Immunogen Information: Recombinant Cubilin (CUBN)

products categories :

FITC Antibody

ncbi gi num :

16758040

ncbi acc num :

NP_445784.1

ncbi gb acc num :

NM_053332.2

uniprot acc num :

O70244

ncbi pathways :

Cobalamin (Cbl, Vitamin B12) Transport And Metabolism Pathway (935625); Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway (936237); Defective BTD Causes Biotidinase Deficiency Pathway (936252); Defective CD320 Causes Methylmalonic Aciduria Pathway (936249); Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway (936238); Defective GIF Causes Intrinsic Factor Deficiency Pathway (936241); Defective HLCS Causes Multiple Carboxylase Deficiency Pathway (936251); Defective LMBRD1 Causes Methylmalonic Aciduria And Homocystinuria Type CblF Pathway (936240); Defective MMAA Causes Methylmalonic Aciduria Type CblA Pathway (936247); Defective MMAB Causes Methylmalonic Aciduria Type CblB Pathway (936246)

ncbi summary :

receptor for intrinsic factor (IF)-cobalamin; involved in vitamin B12 uptake (cyanocobalamin) [RGD, Feb 2006]

uniprot summary :

CUBN: Cotransporter which plays a role in lipoprotein, vitamin and iron metabolism, by facilitating their uptake. Binds to ALB, MB, Kappa and lambda-light chains, TF, hemoglobin, GC, SCGB1A1, APOA1, high density lipoprotein, and the GIF-cobalamin complex. The binding of all ligands requires calcium. Serves as important transporter in several absorptive epithelia, including intestine, renal proximal tubules and embryonic yolk sac. Interaction with LRP2 mediates its trafficking throughout vesicles and facilitates the uptake of specific ligands like GC, hemoglobin, ALB, TF and SCGB1A1. Interaction with AMN controls its trafficking to the plasma membrane and facilitates endocytosis of ligands. May play an important role in the development of the peri-implantation embryo through internalization of APOA1 and cholesterol. Binds to LGALS3 at the maternal-fetal interface. Defects in CUBN are a cause of recessive hereditary megaloblastic anemia 1 (RH-MGA1); also known as MGA1 Norwegian type or Imerslund-Grasbeck syndrome (I-GS). RH-MGA1 is due to selective malabsorption of vitamin B12. Defects in vitamin B12 absorption lead to impaired function of thymidine synthase. As a consequence DNA synthesis is interrupted. Rapidly dividing cells involved in erythropoiesis are particularly affected. Cellular Component: Golgi apparatus; lysosomal lumen; protein complex; lysosomal membrane; brush border membrane; endoplasmic reticulum; coated pit; membrane; endocytic vesicle; apical part of cell; cytoplasm; apical plasma membrane; endosome membrane; endosome; brush border. Molecular Function: identical protein binding; protein homodimerization activity; hemoglobin binding; cobalamin transporter activity; calcium ion binding; receptor activity; cobalamin binding. Biological Process: cholesterol metabolic process; receptor-mediated endocytosis; vitamin metabolic process; in utero embryonic development; lipoprotein transport; cobalamin transport; hemoglobin import; response to nutrient

size1 :

0.1 mg

price1 :

265 USD

size2 :

0.2 mg

price2 :

410

size3 :

1 mg

price3 :

985

more info or order :

MyBioSource product webpage