catalog number :
MBS2013065
products full name :
Polyclonal Antibody to Angiopoietin Like Protein 8 (ANGPTL8)
products short name :
Angiopoietin Like Protein 8 (ANGPTL8)
other names :
angiopoietin-related protein 2; Angiopoietin-related protein 2; angiopoietin-related protein 2; angiopoietin-like protein 2; angiopoietin-like 2; Angiopoietin-like protein 2
products gene name :
ANGPTL8
other gene names :
ANGPTL2; ANGPTL2; ARP2; HARP; ARP2
uniprot entry name :
ANGL2_HUMAN
sequence :
The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MGGPELA QHEELTLLFH GTLQLGQALN GVYRTTEGRL TKARNSLGLY GRTIELLGQE VSRGRDAAQE LRASLLETQM EEDILQLQAE ATAEVLGEVA QAQKVLRDSV QRLEVQLRSA WLGPAYREFE VLKAHADKQS HILWALTGHV QRQRREMVAQ QHRLRQIQER LHTAAL
specificity :
The antibody is a rabbit polyclonal antibody raised against ANGPTL8. It has been selected for its ability to recognize ANGPTL8 in immunohistochemical staining and western blotting.
purity :
Affinity Chromatography
form :
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
storage stability :
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
tested application :
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
app notes :
Western Blot: 1:50-400. Immunocytochemistry in formalin fixed cells: 1:50-500. Immunohistochemistry in formalin fixed frozen section: 1:50-500. Immunohistochemistry in paraffin section: 1:10-100. ELISA: 1:100-200.
other info1 :
Immunogen: Recombinant ANGPTL8 (Met24~Leu196) with two N-terminal Tags, Hist-tag and T7-tag expressed in E Coli
other info2 :
Quality Control: Content: The quality control contains recombinant ANGPTL8 (Met24~Leu196) disposed in loading buffer. Usage: 10ul per well when 3,3'-Diaminobenzidine (DAB) as the substrate. 5ul per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control. Note used fro other purposes. Loading Buffer: 100mM Tris (pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.
ncbi acc num :
NP_036230.1
ncbi gb acc num :
NM_012098.2
ncbi mol weight :
57,104 Da
ncbi summary :
Angiopoietins are members of the vascular endothelial growth factor family and the only known growth factors largely specific for vascular endothelium. Angiopoietin-1, angiopoietin-2, and angiopoietin-4 participate in the formation of blood vessels. ANGPTL2 protein is a secreted glycoprotein with homology to the angiopoietins and may exert a function on endothelial cells through autocrine or paracrine action. [provided by RefSeq, Jul 2008]
uniprot summary :
ANGPTL2: Induces sprouting in endothelial cells through an autocrine and paracrine action. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 9q34. Cellular Component: extracellular space. Molecular Function: receptor binding. Biological Process: multicellular organismal development