catalog number :
MBS2012482
products type :
Recombinant Protein
products full name :
Recombinant Secretory Leukocyte Peptidase Inhibitor (SLPI)
products short name :
Secretory Leukocyte Peptidase Inhibitor (SLPI)
other names :
antileukoproteinase; Antileukoproteinase; antileukoproteinase; ALP; secretory leukocyte protease inhibitor; secretory leukocyte peptidase inhibitor; Secretory leukocyte protease inhibitor
products gene name :
SLPI
other gene names :
Slpi; Slpi; ALP
uniprot entry name :
SLPI_MOUSE
sequence :
WTVEGGKNDA IKIGACPAKK PAQCLKLEKP QCRTDWECPG KQRCCQDACG SKCVNPVPIR KPVWRKPGRC VKTQARCMML NPPNVCQRDG QCDGKYKCCE GICGKVCLPP M
MHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTD
DDDKAMADIGSEF-P
The target protein is fused with two N-terminal Tags, His-tag and S-tag, its sequence is listed below.
form :
Supplied as lyophilized form in 10mM PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and preservative.
storage stability :
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
tested application :
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
other info1 :
Organism: Mus musculus (Mouse). Expression System: Prokaryotic expression. Residues: Pro20~Met131 (Accession # P97430) with two N-terminal Tags, His-tag and S-tag
other info2 :
Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Reconstitution: Reconstitute in sterile ddH2O. Predicted isoelectric point: 8.7
. Predicted Molecular Mass: 18.1kDa
ncbi acc num :
NP_035544.1
ncbi gb acc num :
NM_011414.3
uniprot summary :
SLPI: Acid-stable proteinase inhibitor with strong affinities for trypsin, chymotrypsin, elastase, and cathepsin G. May prevent elastase-mediated damage to oral and possibly other mucosal tissues. Protein type: Secreted, signal peptide; Inhibitor; Secreted. Cellular Component: extracellular matrix; extracellular region. Molecular Function: serine-type endopeptidase inhibitor activity; enzyme binding; protease inhibitor activity; endopeptidase inhibitor activity. Biological Process: negative regulation of viral genome replication; negative regulation of peptidase activity; negative regulation of protein binding