catalog number :
MBS2012459
products type :
Recombinant Protein
products full name :
Recombinant Islet Cell Autoantigen 1 (ICA1)
products short name :
Islet Cell Autoantigen 1 (ICA1)
other names :
islet cell autoantigen 1 isoform 2; Islet cell autoantigen 1; islet cell autoantigen 1; p69; ICAp69; islet cell autoantigen p69; 69 kDa islet cell autoantigen; islet cell autoantigen 1, 69 kDa; islet cell autoantigen 1; 69 kDa islet cell autoantigen; ICA69; Islet cell autoantigen p69
products gene name :
ICA1
other gene names :
Ica1; Ica1; 69kDa; ICA69; Icap69; ICA69; ICAp69; p69
uniprot entry name :
ICA69_MOUSE
sequence :
LQDRFAQDKS VVNKMQQKYW ETKQAFIKAT GKKEDEHVVA SDADLDAKLE LFHSIQRTCL DLSKAIVLYQ KRICFLSQEE NELGKFLRSQ GFQDKTRAGK MMQATGKALC FSSQQRLALR NPLCRFHQEV ETFRHRAISD TWLTVNRMEQ YRTEYRGALL WMKDVSQELD PDLYKQMEKF RKVQTQVRLA KKNFDKLKMD VCQKVDLLGA SRCNLLSHML ATYQTTLLHF WEKTSHTMAA IHESFKG
MHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTD
DDDKAMADIGSEF-MSGHKCYSWE
The target protein is fused with two N-terminal Tags, His-tag and S-tag, its sequence is listed below.
form :
Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1 mM EDTA, 1 mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
storage stability :
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
tested application :
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). (May be suitable for use in other assays to be determined by the end user.)
other info1 :
Organism: Mus musculus (Mouse). Expression System: Prokaryotic expression. Residues: Met1~Gly257 (Accession # P97411) with two N-terminal Tags, His-tag and S-tag
other info2 :
Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4. Predicted isoelectric point: 8.8. Predicted Molecular Mass: 35.9kDa as determined by SDS-PAGE reducing conditions.
products description :
About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.
ncbi acc num :
NP_001239195.1
ncbi gb acc num :
NM_001252266.1
ncbi mol weight :
26&32&37kDa as determined by SDS-PAGE reducing conditions.
ncbi pathways :
Type I Diabetes Mellitus Pathway (83292); Type I Diabetes Mellitus Pathway (507)
uniprot summary :
ICA1: May play a role in neurotransmitter secretion. Protein type: Unknown function. Cellular Component: Golgi membrane; Golgi apparatus; Golgi stack; synaptic vesicle membrane; membrane; perinuclear region of cytoplasm; secretory granule membrane; dendrite; cytoplasm; synapse; cytoplasmic vesicle; cell junction; cytosol. Molecular Function: protein domain specific binding. Biological Process: transport; neurotransmitter transport; regulation of protein homodimerization activity; regulation of neurotransmitter secretion