Recombinant Laminin Alpha 4 (LAMa4) | MyBioSource MBS2012393 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Laminin Alpha 4 (LAMa4)

catalog :

MBS2012393

quantity :

0.01 mg

price :

160 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2012393

products type :

Recombinant Protein

products full name :

Recombinant Laminin Alpha 4 (LAMa4)

products short name :

Laminin Alpha 4 (LAMa4)

other names :

laminin subunit alpha-4 isoform 1; Laminin subunit alpha-4; laminin subunit alpha-4; laminin alpha 4 chain; laminin, alpha 4; Laminin-14 subunit alpha; Laminin-8 subunit alpha; Laminin-9 subunit alpha

products gene name :

LAMa4

other gene names :

LAMA4; LAMA4; LAMA3; CMD1JJ; LAMA4*-1

uniprot entry name :

LAMA4_HUMAN

host :

E Coli

reactivity :

Homo sapiens (Human)

sequence length :

1823

sequence :

The sequence of the target protein is listed below. IQVSMMFDG QSAVEVHSRT SMDDLKAFTS LSLYMKPPVK RPELTETADQ FILYLGSKNA KKEYMGLAIK NDNLVYVYNL GTKDVEIPLD SKPVSSWPAY FSIVKIERVG KHGKVFLTVP SLSSTAEEKF IKKGEFSGDD SLLDLDPEDT VFYVGGVPSN FKLPTSLNLP GFVGCLELAT LNNDVISLYN FKHIYNMDPS TSVPCARDK

purity :

> 95%

form :

Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.

storage stability :

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

tested application :

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

other info1 :

Residues: Ile832~Lys1039 (Accession # Q16363) with N-terminal His-Tag. Subcellular Location: Secreted, extracellular space, extracellular matrix, basement membrane. Predicted isoelectric point: 6.0. Predicted Molecular Mass: 24.6kDa. Accurate Molecular Mass: 29 kDa as determined by SDS-PAGE reducing conditions.

other info2 :

Endotoxin Level: <1.0EU per 1ug(determined by the LAL method). Reconstitution: Reconstitute in sterile ddH2O.

ncbi gi num :

380503843

ncbi acc num :

NP_001098676.2

ncbi gb acc num :

NM_001105206.2

uniprot acc num :

Q16363

ncbi pathways :

African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); ECM Proteoglycans Pathway (833812); ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479); Extracellular Matrix Organization Pathway (576262); FOXM1 Transcription Factor Network Pathway (137935); Focal Adhesion Pathway (198795)

ncbi summary :

Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the alpha chain isoform laminin, alpha 4. The domain structure of alpha 4 is similar to that of alpha 3, both of which resemble truncated versions of alpha 1 and alpha 2, in that approximately 1,200 residues at the N-terminus (domains IV, V and VI) have been lost. Laminin, alpha 4 contains the C-terminal G domain which distinguishes all alpha chains from the beta and gamma chains. The RNA analysis from adult and fetal tissues revealed developmental regulation of expression, however, the exact function of laminin, alpha 4 is not known. Tissue-specific utilization of alternative polyA-signal has been described in literature. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Aug 2011]

uniprot summary :

LAMA4: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Extracellular matrix; Secreted. Chromosomal Location of Human Ortholog: 6q21. Cellular Component: extracellular matrix; laminin-1 complex; extracellular region; basement membrane; basal lamina. Molecular Function: protein binding; extracellular matrix structural constituent; receptor binding. Biological Process: regulation of cell adhesion; extracellular matrix organization and biogenesis; regulation of embryonic development; cell adhesion; regulation of cell migration. Disease: Cardiomyopathy, Dilated, 1jj

size1 :

0.01 mg

price1 :

160 USD

size2 :

0.05 mg

price2 :

320

size3 :

0.1 mg

price3 :

495

more info or order :

MyBioSource product webpage