catalog number :
MBS2012379
products type :
Recombinant Protein
products full name :
Recombinant Neuromedin B (NMB)
products short name :
Neuromedin B (NMB)
other names :
neuromedin-B; Neuromedin-B; neuromedin-B; neuromedin B
other gene names :
Nmb; Nmb; 3110023K12Rik
uniprot entry name :
NMB_MOUSE
sequence :
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below. MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF- SWL LRGLLLFALF ASGVAPFNWD LPEPRSRASK IRVHPRGNLW ATGHFMGKKS LEPPSLSLVG TAPPNTPRDQ RLQLSHDLLR ILLRKKALGM NFSGPAPPIQ YRRL
form :
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
storage stability :
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
tested application :
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
other info1 :
Organism: Mus musculus (Mouse). Expression System: Prokaryotic expression. Residues: Ser8~Leu114 (Accession # Q9CR53) with two N-terminal Tags, His-tag and GST-tag
other info2 :
Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4. Subcellular Location: Secreted. Predicted isoelectric point: 9.2
products description :
About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.
ncbi acc num :
NP_080799.1
ncbi gb acc num :
NM_026523.3
ncbi mol weight :
39.1kDa
ncbi pathways :
Class A/1 (Rhodopsin-like Receptors) Pathway (926991); G Alpha (q) Signalling Events Pathway (927024); GPCR Downstream Signaling Pathway (927020); GPCR Ligand Binding Pathway (926990); Gastrin-CREB Signalling Pathway Via PKC And MAPK (927038); Peptide Ligand-binding Receptors Pathway (926992); Signal Transduction Pathway (926897); Signaling By GPCR Pathway (926989)
uniprot summary :
NMB: Stimulates smooth muscle contraction in a manner similar to that of bombesin. Belongs to the bombesin/neuromedin-B/ranatensin family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted. Cellular Component: neuron projection; extracellular region; cytosol. Molecular Function: neuromedin B receptor binding. Biological Process: elevation of cytosolic calcium ion concentration; neuropeptide signaling pathway; negative regulation of hormone secretion; positive regulation of cell proliferation; positive regulation of hormone secretion; glucose homeostasis; arachidonic acid secretion