protein

Recombinant Amyloid Precursor Protein (APP)

MBS2012348

0.01 mg

200 USD

Recombinant Protein

Recombinant Amyloid Precursor Protein (APP)

Amyloid Precursor Protein (APP)

amyloid beta A4 protein isoform 1; Amyloid beta A4 protein; amyloid beta A4 protein; appican; protease nexin II; amyloidogenic glycoprotein; alzheimer disease amyloid A4 protein homolog; amyloid beta (A4) precursor protein; ABPP; APP; Alzheimer disease amyloid A4 protein homolog; Amyloidogenic glycoprotein; AGCleaved into the following 14 chains:N-APPSoluble APP-alpha; S-APP-alphaSoluble APP-beta; S-APP-betaC99; Alternative name(s):; APP-C99Beta-amyloid protein 42; Alternative name(s):; Beta-APP42Beta-amyloid protein 40; Alternative name(s):; Beta-APP40C83P3(42)P3(40)C80Gamma-secretase C-terminal fragment 59; Alternative name(s):; APP-C59; Amyloid intracellular domain 59; AID(59); Gamma-CTF(59)Gamma-secretase C-terminal fragment 57; Alternative name(s):; APP-C57; Amyloid intracellular domain 57; AID(57); Gamma-CTF(57)Gamma-secretase C-terminal fragment 50; Alternative name(s):; Amyloid intracellular domain 50; AID(50); Gamma-CTF(50)

APP

App; App; Ag; Abpp; Adap; Cvap; Abeta; betaApp; E030013M08Rik; APP; AG; S-APP-alpha; S-APP-beta; AID(59); AID(57); AID(50)

A4_MOUSE

E Coli

Mus musculus (Mouse)

770

The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSGS-DAEFGHDSG FEVRHQKLVF FAEDVGSNKG AIIGLMVGGV VIATVIVITL VMLKKKQYTS IHHGVVEVDA AVTPEERHLS KMQQNGYENP TYKFFEQMQN

> 95%

Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

Expression System: Prokaryotic expression. Residues: Asp672~Asn770 (Accession # P12023) with N-terminal His-Tag. Predicted isoelectric point: 6.4. Accurate Molecular Mass: 18kDa as determined by SDS-PAGE reducing conditions.

Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Reconstitution: Reconstitute in ddH2O.

About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.

311893401

NP_001185752.1

NM_001198823.1

P12023

12.3kDa

Activated TLR4 Signalling Pathway (926081); Advanced Glycosylation Endproduct Receptor Signaling Pathway (926102); Alzheimer's Disease Pathway (83294); Alzheimer's Disease Pathway (509); Amyloids Pathway (926240); Class A/1 (Rhodopsin-like Receptors) Pathway (926991); Cytosolic Sensors Of Pathogen-associated DNA Pathway (926112); DEx/H-box Helicases Activate Type I IFN And Inflammatory Cytokines Production Pathway (926120); Delta-Notch Signaling Pathway (198316); Disease Pathway (926229)

APP: a cell surface receptor that influences neurite growth, neuronal adhesion and axonogenesis. Cleaved by secretases to form a number of peptides, some of which bind to the acetyltransferase complex Fe65/TIP60 to promote transcriptional activation. The Abeta peptide is released from the cell, its extracellular deposition and accumulation form the main components of amyloid plaques in Alzheimer s disease. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis. Can promote transcription activation through binding to Fe65-Tip60 and inhibits Notch signaling through interaction with Numb. Couples to apoptosis-inducing pathways such as those mediated by G(O) and JIP. Inhibits G(O) alpha ATPase activity. Acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1. Involved in copper homeostasis/oxidative stress through copper ion reduction. In vitro, copper-metallated APP induces neuronal death directly or is potentiated through Cu(2+)-mediated low-density lipoprotein oxidation. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I and IV. Induces a RAGE-dependent pathway that activates p38 MAPK, resulting in internalization of amyloid-beta peptide and leading to mitochondrial dysfunction in cultured cortical neurons. Provides Cu(2+) ions for GPC1 which are required for release of nitric oxide (NO) and subsequent degradation of the heparan sulfate chains on GPC1. Binds, via its C-terminus, to the PID domain of several cytoplasmic proteins, including APBB family members, the APBA family, JIP1, SHC1 and, NUMB and DAB1. Binding to DAB1 inhibits its serine phosphorylation. Associates with microtubules in the presence of ATP and in a kinesin-dependent manner. Amyloid beta-42 binds nAChRA7 in hippocampal neurons. Beta-amyloid associates with HADH2. Soluble APP binds, via its N-terminal head, to FBLN1. Expressed in all fetal tissues examined with highest levels in brain, kidney, heart and spleen. Weak expression in liver. In adult brain, highest expression found in the frontal lobe of the cortex and in the anterior perisylvian cortex- opercular gyri. Moderate expression in the cerebellar cortex, the posterior perisylvian cortex-opercular gyri and the temporal associated cortex. Weak expression found in the striate, extra- striate and motor cortices. Expressed in cerebrospinal fluid, and plasma. 10 isoforms of the human protein are produced by alternative splicing. Isoform APP695 is the predominant form in neuronal tissue, isoform APP751 and isoform APP770 are widely expressed in non- neuronal cells. Isoform APP751 is the most abundant form in T-lymphocytes. Appican is expressed in astrocytes. The splice isoforms that contain the BPTI domain possess protease inhibitor activity. Belongs to the APP family. Protein type: Cell surface; Membrane protein, integral; Apoptosis; Transcription factor; Receptor, misc. Cellular Component: Golgi apparatus; extracellular space; cell surface; smooth endoplasmic reticulum; integral to membrane; terminal button; coated pit; intercellular junction; ER to Golgi transport vesicle; lipid raft; ciliary rootlet; membrane; perinuclear region of cytoplasm; nuclear envelope lumen; cytoplasm; cytoplasmic vesicle; intracellular; neuromuscular junction; receptor complex; endosome; neuron projection; intracellular membrane-bound organelle; cell; apical part of cell; axon; plasma membrane; spindle midzone. Molecular Function: serine-type endopeptidase inhibitor activity; heparin binding; identical protein binding; protease activator activity; protease inhibitor activity; metal ion binding; PTB domain binding; acetylcholine receptor binding; protein binding; enzyme binding; DNA binding; transition metal ion binding; receptor binding. Biological Process: extracellular matrix organization and biogenesis; apoptosis; adult locomotory behavior; negative regulation of peptidase activity; locomotory behavior; mRNA polyadenylation; positive regulation of mitotic cell cycle; protein amino acid phosphorylation; regulation of translation; synaptic growth at neuromuscular junction; forebrain development; dendrite development; visual learning; collateral sprouting in the absence of injury; neuromuscular process controlling balance; cell adhesion; protein homooligomerization; neurite development; cholesterol metabolic process; nervous system development; Notch signaling pathway; cellular copper ion homeostasis; regulation of epidermal growth factor receptor activity; mating behavior; axon cargo transport; regulation of multicellular organism growth; endocytosis; axon midline choice point recognition; smooth endoplasmic reticulum calcium ion homeostasis; negative regulation of neuron differentiation; neuron apoptosis; suckling behavior; axonogenesis; regulation of gene expression; ionotropic glutamate receptor signaling pathway; regulation of synapse structure and activity; regulation of protein binding; positive regulation of transcription from RNA polymerase II promoter; response to oxidative stress; neuron remodeling

0.01 mg

200 USD

0.05 mg

430

0.1 mg

665